SKRINING AKTIVITAS ANTIOKSIDAN FRAKSI-FRAKSI DARI TUMBUHAN OBAT MASYARAKAT KAYU AGUNG YANG TERSELEKSI

SETIA, HARDIYANTI and Elfita, Elfita and Shaum, Shiyan (2022) SKRINING AKTIVITAS ANTIOKSIDAN FRAKSI-FRAKSI DARI TUMBUHAN OBAT MASYARAKAT KAYU AGUNG YANG TERSELEKSI. Undergraduate thesis, Sriwijaya University.

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Abstract

Free radicals in the body can cause various degenerative diseases such as cancer, rheumatism and diabetes, to protect the body from the effects of free radicals, an antioxidant compound is needed. This study aimed to determine the antioxidant activity, total flavonoid content, total phenolic content and characterization of the seed and leaf fractions of avocado (Persea Americana Mill), asoka leaf (Ixora coccinea L), leaf and bark of matoa (Pometia pinnata). The maceration method was carried out by graded maceration on n-hexane, ethyl acetate and methanol as solvents. Determination of antioxidant activity was carried out on the fractions using the DPPH method. Total flavonoid and total phenolic levels using UV-Vis spectrophotometry. The results showed that the n-hexane fraction had very strong antioxidant activity from avocado seeds and matoa bark at 35.82 g/mL and 46.74 g/mL, while the ethyl acetate and methanol fractions had very strong antioxidant activity ( <50 g/mL) for all samples. The total flavonoid and phenolic content which has the largest content is found in the sample of asoka leaf methanol fraction of 12.09 mgQE/g and 92.84 mgGAE/g. Duncan IC50 post hoc test results showed that the n-hexane fraction of avocado leaves & avocado seeds was significantly different. Matoa leaves & bark were significantly different. The ethyl acetate fraction of avocado leaves & avocado seeds was significantly different. Matoa leaves & bark were significantly different. Avocado leaf & avocado seed methanol fraction was not significantly different. Matoa leaves & stem bark were not significantly different, for asoka leaves there was a significant difference between the n-hexane fraction with ethyl acetate and methanol, and the characterization of the n-hexane, ethyl acetate and methanol fractions for each parameter met the requirements of the Ministry of Health 2017.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Ethyl acetate, N-hexane, Methanol, DPPH
Subjects: R Medicine > RS Pharmacy and materia medica > RS189-190 Assay methods. Standardization. Analysis
Divisions: 08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User: Setia Hardiyanti
Date Deposited: 06 Jun 2022 05:43
Last Modified: 06 Jun 2022 07:53
URI: http://repository.unsri.ac.id/id/eprint/71742

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