OPTIMASI NANOEMULSI VIRGIN COCONUT OIL (VCO) DENGAN METODE DESAIN FAKTORIAL DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS SECARA IN VITRO

BIHURININ, AZZAHRA HITHTHAH BAMA and Miksusanti, Miksusanti and Apriani, Elsa Fitria (2022) OPTIMASI NANOEMULSI VIRGIN COCONUT OIL (VCO) DENGAN METODE DESAIN FAKTORIAL DAN UJI AKTIVITAS ANTIBAKTERI TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS SECARA IN VITRO. Undergraduate thesis, Sriwijaya University.

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Abstract

Virgin Coconut Oil (VCO) contains lauric acid which has the potential as an antibacterial, used against Staphylococcus aureus. The preparation of VCO nanoemulsion was carried out to increase its antibacterial effect. This research was conducted to optimize the VCO nanoemulsion preparation and to determine its antibacterial activity against Staphylococcus aureus bacteria compared to VCO without a nanoemulsion. Optimization was carried out using the 22 factorial design method through Design Expert 13® on the response of pH, specific gravity, percent transmittance, particle size, and particle distribution. The optimum formula of the VCO nanoemulsion that has been obtained was evaluated by centrifugation test, cycling test, determination of the diameter of the inhibition zone by disc diffusion method, minimum inhibitory concentration (MIC), and minimum kill concentration (MBC). Optimum formulation of nanoemulsion preparation VCO at a emulsifier concentration using Tween 80 40% and PEG 400 26%. The centrifugation test showed no phase separation. The cycling test showed a decrease in pH that has no significant difference (p>0.05) from 6.23 ± 0.01 to 6.21 ± 0.01. The antibacterial activity test showed a significant difference (p<0.05) between the optimum formula of VCO nanoemulsion and VCO without nanoemulsion, but both were equally effective in inhibiting bacterial growth with an inhibition zone diameter of 24.77 mm ± 1.662328 on VCO nanoemulsion and VCO nanoemulsion. 16.73 ± 2.003331 on VCO without nanoemulsion. The MIC test results showed the value of VCO nanoemulsion at a concentration of 1250 ppm while VCO without nanoemulsion at a concentration of 2500 ppm. The MBC test results showed the nanoemulsion MBC value at a concentration of 2500 ppm, while the VCO without nanoemulsion did not have a MBC value of several concentration tested. Keywords: VCO, nanoemulsion, optimization, antibacterial.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	VCO, nanoemulsi, optimasi, antibakteri.
Subjects:	Q Science > QR Microbiology > QR75-99.5 Bacteria R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic) R Medicine > RS Pharmacy and materia medica > RS192-199 Pharmaceutical technology
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Azzahra Hiththah Bama Bihurinin
Date Deposited:	08 Aug 2022 08:43
Last Modified:	08 Aug 2022 08:43
URI:	http://repository.unsri.ac.id/id/eprint/76465

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