KORELASI AKTIVITAS PEROKSIDASE DAN POLIFENOL OKSIDASE DENGAN AKUMULASI LOGAM BERAT Cu DAN Pb PADA Avicennia marina (Forssk) Vierh DI PULAU PAYUNG, SUMATERA SELATAN

NINGSI, RISKI TRI LARAS and Juswardi, Juswardi and Sarno, Sarno (2022) KORELASI AKTIVITAS PEROKSIDASE DAN POLIFENOL OKSIDASE DENGAN AKUMULASI LOGAM BERAT Cu DAN Pb PADA Avicennia marina (Forssk) Vierh DI PULAU PAYUNG, SUMATERA SELATAN. Undergraduate thesis, Sriwijaya University.

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Abstract

Pencemaran logam berat yang terjadi di Pulau Payung erat kaitannya dengan kegiatan perindustrian. Salah satu upaya untuk mengatasi permasalahan logam berat adalah dengan cara fitoremediasi. Avicennia marina (Forrsk) Vierh mempunyai kemampuan beradaptasi terhadap pencemaran logam berat. Respons fisiologis A. marina terhadap stress logam berat menyebabkan terjadinya perubahan aktivitas enzim radikal bebas terutama peroksidase (PO) dan polifenol oksidase (PPO) sehingga dilakukan penelitian yang bertujuan mengetahui hubungan PO dan PPO dengan akumulasi logam berat Cu dan Pb pada A. marina di Kawasan mangrove Pulau Payung, Sumatera Selatan. Proses pengambilan sampel menggunakan metode purposive sampling. Ekstraksi protein dengan sentrifugasi pada kecepatan 12.500 g suhu 4°C selama 20 menit. Pengukuran kadar protein total dengan reagen biuret (Biosystem 2000) dengan standar BSA. dan aktivitas PO dan PPO menggunakan pereaksi pirogalol secara spektrogitometri masing pada panjang gelombang 420 nm dan 240 nm.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Avicennia marina (Forrsk) Vierh, Fitoremediasi, Logam Berat, Peroksidase, Polifenol Oksidase
Subjects: Q Science > QP Physiology > QP1-(981) Physiology
Divisions: 08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User: Riski Tri Laras Ningsi
Date Deposited: 19 Sep 2022 05:24
Last Modified: 19 Sep 2022 05:24
URI: http://repository.unsri.ac.id/id/eprint/78544

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