EKSPLORASI BAKTERI SELULOLITIK DARI USUS LARVA LALAT TENTARA HITAM (Hermetia illucens L.) YANG DIBERI PAKAN BUNGKIL INTI SAWIT

RAHMATULLAH, REZA and Muharni, Muharni (2022) EKSPLORASI BAKTERI SELULOLITIK DARI USUS LARVA LALAT TENTARA HITAM (Hermetia illucens L.) YANG DIBERI PAKAN BUNGKIL INTI SAWIT. Undergraduate thesis, Sriwijaya University.

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Abstract

The black soldier fly (BSF) larvae is renowned as bioconversion agent of various types organic waste. Gut microbes play an essential role in digestion of complex substrates by producing various enzymes inside larvae bodies. One of various enzymes is cellulase which hydrolyze cellulose polysaccharide into glucoses which is produced by cellulolytic bacteria. Palm kernel meal (PKM), larvae feedstock containing cellulose polysaccharide, is able to be converted by BSF larvae which is assumed that it is impacted on cellulolytic bacteria within gut larvae. The intention of this study is to screen cellulolytic bacteria, examine the overall reducing sugar with DNS reagent method and identify cellulolytic bacteria from their characterization and biochemical tests. Based on the result of experiment, Four bacteria isolates labelled as MB1, MB6, MB7, and MB8 were able to form transparent zone within carboxylmethyl cellulose (CMC) agar plate. MB1 and MB8 are respectively the highest and the lowest of cellulase activity isolate which each value is 7.13 U/ml and 5.77 U/ml. Four cellulolytic bacteria isolates is identified as Listeria sp. for MB1 and Enterococcus sp. for MB6, MB7 and MB8.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	BSF larvae, Cellulase, Cellulolytic bacteria, Palm Kernel Meal
Subjects:	Q Science > QR Microbiology Q Science > QR Microbiology > QR171 Microorganisms in the animal body Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Users 25002 not found.
Date Deposited:	30 Sep 2022 08:14
Last Modified:	30 Sep 2022 08:14
URI:	http://repository.unsri.ac.id/id/eprint/80270

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