EFEK INHIBISI EKSTRAK DAN FRAKSI NONPOLAR ETIL ASETAT DAUN BENALU KERSEN TERHADAP ENZIM XANTIN OKSIDASE

LIE, CAROLYN and Sinulingga, Sadakata and Subandrate, Subandrate (2022) EFEK INHIBISI EKSTRAK DAN FRAKSI NONPOLAR ETIL ASETAT DAUN BENALU KERSEN TERHADAP ENZIM XANTIN OKSIDASE. Undergraduate thesis, Sriwijaya University.

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Abstract

Introduction: Kersen plant (Muntingia calabura L.) is known to contains flavonoids, triterpenoids, tannin and alkaloids which have ability to inhibit xanthine oxidase enzyme in vivo and in vitro. These metabolite compounds is worked by binding to the active site of xanthine oxidase enzyme thereby preventing substrate to binding with xanthine oxidase enzyme. Benalu kersen leaves (Dendrophthoe pentandra (L). Miq) contains metabolite compound that are similar with kersen leaves due to their host plant. Theirfore further research was carried out regarding the potential of the extract and nonpolar fraction ethyl asetate benalu kersen leaves. Method: The viscous simplicia of benalu kersen leaves was extracted by the maceration method using ethyl acetate solvent then followed by fractionation by the liquid-liquid extraction method with a mixture of n-hexane and ethyl acetate solvents divided into 5 different concentrations. The xanthine oxidase enzyme inhibition test was measured on the extract, fraction and allopurinol positive control. The UV-Vis spectrophotometer with a wavelength of 293 nm measures the absorbance value of the sample and then calculates the inhibition percentage of the xanthine oxidase enzyme. The percentage inhibition results were calculated using the linear regression analysis equation to obtain IC50 values which could be classified into three groups, namely very active, active and inactive. Result: Extracts and several concentrations of certain solvent mixtures of the nonpolar ethyl acetate fraction of benalu kersen leaves contain secondary metabolites of alkaloids, flavonoids, triterpenoids and tannins. IC50 value of extract and nonpolar ethyl acetate fraction of benalu kersen leaves with a ratio of n-hexane and ethyl acetate (9:1), (7:3), (5:5), (3:7), and (1:9) solvents respectively 9.85 ppm, 12.06 ppm, 6.20 ppm, 4.77 ppm, 6.52 ppm, 8.48 ppm which all belong to the very active group as xanthine oxidase inhibitors. The IC50 value in the allopurinol positive control was 1.33 ppm. Keywords: Ethyl acetate extract, nonpolar fraction of ethyl acetate extract, (Dendrophthoe pentandra (L). Miq), secondary metabolite compounds, inhibition of the xanthine oxidase enzyme.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Ethyl acetate extract, nonpolar fraction of ethyl acetate extract, (Dendrophthoe pentandra (L). Miq), secondary metabolite compounds, inhibition of the xanthine oxidase enzyme.
Subjects:	Q Science > QD Chemistry > QD415-436 Biochemistry
Divisions:	04-Faculty of Medicine > 11201-Medicine (S1)
Depositing User:	Carolyn Lie
Date Deposited:	20 Dec 2022 03:40
Last Modified:	20 Dec 2022 03:40
URI:	http://repository.unsri.ac.id/id/eprint/84128

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