EFEKTIVITAS FRAKSI POLAR EKSTRAK ETIL ASETAT DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM XANTIN OKSIDASE

FAHIRA, KARINA NURUL and Parisa, Nita and Theodorus, Theodorus (2022) EFEKTIVITAS FRAKSI POLAR EKSTRAK ETIL ASETAT DAUN KERSEN (Muntingia calabura L.) SEBAGAI INHIBITOR ENZIM XANTIN OKSIDASE. Undergraduate thesis, Sriwijaya University.

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Abstract

Background: Xanthine oxidase is an enzyme that catalyzes the formation of uric acid. It is known that kersen leaves contains secondary metabolites such as flavonoids, which have antihyperuricemia potential. This study aims to determine the efficacy of the polar fraction of ethyl acetate extract of kersen leaves (Muntingia calabura Linn) as an antihyperuricemia tested in vitro. Methods: Experimental in vitro study has been conducted from August to October 2022 at the Faculty of Medicine, University of Sriwijaya, Palembang. There are 7 groups consisting of kersen leaf ethyl acetate extract, ethanol fraction 1;2;3;4;5, and allopurinol. Percent inhibition results were analyzed by Independent T Test and LSD and using SPSS version 25. Results: The polar fraction of kersen leaf ethyl acetate extract with an average weight of 3.86 grams contains secondary metabolites such as alkaloids, triterpenoids, flavonoids and tannins. The IC50 value of the ethyl acetate extract of kersen leaves was 11.02 ppm and the positive control (allopurinol) was 17,16 ppm which classified as very strong in inhibiting the xanthine oxidase enzyme. The polar fraction of ethyl acetate extract of kersen leaves respectively according to the ethyl acetate solvent mixture: 96% ethanol (9:1), (7:3), (5:5), (3:7), and (1:9) have IC50 values of 49,51 ppm, 50,77 ppm, 42,85 ppm, 40,02 ppm, and 69,82 ppm which are classified as having strong abilities to inhibit the xanthine oxidase enzyme. Conclusion: It can be concluded that ethyl acetate extract of kersen leaves is more effective than the polar fraction of ethyl acetate of kersen leaves and allopurinol in inhibiting the xanthine oxidase enzyme. Keywords: Kersen leaves (Muntingia calabura Linn), xanthine oxidase enzyme inhibition, phytochemical tests, polar fraction of ethyl acetate extract, in vitro

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Daun Kersen (Muntingia calabura Linn), inhibisi enzim xantin oksidase, uji fitokimia, fraksi polar ekstrak etil asetat, in vitro
Subjects:	R Medicine > RM Therapeutics. Pharmacology > RM1-950 Therapeutics. Pharmacology
Divisions:	04-Faculty of Medicine > 11201-Medicine (S1)
Depositing User:	Karina Nurul Fahira
Date Deposited:	20 Dec 2022 07:05
Last Modified:	20 Dec 2022 07:05
URI:	http://repository.unsri.ac.id/id/eprint/84136

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