SCREENING BENIH PADI (ORYZA SATIVA L.) VARIETAS LOKAL SUMATERA SELATAN MENGGUNAKAN LARUTAN PEG 6000 DAN METODE MARKA MOLEKULER (SSR)

ALHAMAMI, ZENDI and Hasmeda, Mery (2023) SCREENING BENIH PADI (ORYZA SATIVA L.) VARIETAS LOKAL SUMATERA SELATAN MENGGUNAKAN LARUTAN PEG 6000 DAN METODE MARKA MOLEKULER (SSR). Undergraduate thesis, Sriwijaya University.

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Abstract

The rice plant (Oryza Sativa L.) is one of the most important plants for the world that is thought to have originated in the indian or indochina area. This study aims to find local South Sumatra varieties of rice that are resistant to drought stress using Polyethylene Glycol (PEG) and PCR-RAPD solutions. This research was conducted at the Agricultural Cultivation laboratory, Faculty of Agriculture, Sriwijaya University, North Indralaya District, Ogan Ilir Regency, South Sumatra. This research starts from August to December 2022. This study used 2 factors, the first factor is Aquades solution as control, PEG with a concentration of 10%, and PEG with a concentration of 20%. The second factor, namely PCR-RAPD, is used by combining PCR techniques with random sequenss primers for the purpose of genome random locus amplication. The parameters observed were sprout length, bud length, main root length, seminar root length, germination percentage, dry weight of shoots, dry weight of roots, vigor indexion and PCR-RAPD amplication results. The results of the PEG study showed that solar rice, golden solar, tehas and short bridges are tolerant of drought stress, followed by DNA amplification which shows the results of DNA bands on each marker against solar rice, golden solar, jambat tehas and short bridges have similarities in DNA bands with comparison varieties that are resistant to drought stress.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Padi Lokal, PEG, PCR-RAPD, Marka Molekuler.
Subjects:	S Agriculture > SB Plant culture > SB113.2-118.46 Seeds. Seed technology S Agriculture > SB Plant culture > SB119-124 Propagation. Including breeding, selection, grafting, air layering
Divisions:	05-Faculty of Agriculture > 54204-Agronomy (Agricultural Sciences) (S1)
Depositing User:	Zendi Alhamami
Date Deposited:	31 Jan 2023 01:57
Last Modified:	31 Jan 2023 02:02
URI:	http://repository.unsri.ac.id/id/eprint/88498

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