skip to main content

Cytotoxic, Antiproliferation, and Necrosis Effects of the n-Hexane Fraction Extract of Gendola Leaf (Basella rubra Linn.)

1Poltekkes Kemenkes Palembang, Indonesia

2Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Sriwijaya, Indonesia

3Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya, Indonesia

Received: 25 Jul 2019; Revised: 15 Nov 2019; Accepted: 17 Dec 2019; Available online: 31 Jan 2019; Published: 31 Jan 2020.
Open Access Copyright 2020 Jurnal Kimia Sains dan Aplikasi under http://creativecommons.org/licenses/by-sa/4.0.

Citation Format:
Cover Image
Abstract
Breast cancer is the second most common cancer in women. Several studies have been conducted on natural materials to see the ability of its activity as a new anticancer compound. The purpose of this study was to determine the cytotoxic activity of Gendola leaf (Basella rubra Linn.) extracts and fractions, in inhibiting proliferation and inducing apoptosis of T47D breast cancer cells. The design of this study was experimental laboratory research using the in-vitro method. Cytotoxic and anti-proliferation tests on T47D cancer were using the MTT method, and the apoptosis test was using the flow cytometry method. Cytotoxic of extracts and fractions of n-hexane Gendola leaves were tested with a concentration series of 1000; 500; 250; 125; 62.5 μg/mL and doxorubicin was studied a series concentration of 0.5; 0.25; 0.125; 0.0625; 0.03125 μg/mL for 24 hours. Antiproliferation test used n-hexane fraction with a concentration series of 1IC50, ½IC50, ¼IC50, and ⅛IC50 with an incubation time of 24 and 48 hours. Apoptosis test utilized n-hexane fraction with a concentration series of 1IC50, ½IC50 with an incubation time of 24 hours. The results showed that the ethanol extract had IC50 424 μg/mL, n-hexane fraction 292 μg/mL. The n-hexane fraction had an anti-proliferation effect at a concentration of 1IC50 within 32 hours but was unable to induce T47D cell apoptosis. These results indicate that the n-hexane fraction of Gendola leaf has a cytotoxic effect, which can inhibit proliferation, that is unable to induce apoptosis but induce necrosis of T47D breast cancer cells.

Note: This article has supplementary file(s).

Fulltext View|Download |  Sertifikat
Sertifikat Persetujuan Etik
Subject
Type Sertifikat
  View (449KB)    Indexing metadata
Keywords: cytotoxic; antiproliferation; apoptosis; Gendola leaf extract and fraction (Basella rubra Linn.); necrosis
Funding: Poltekkes Kemenkes

Article Metrics:

Article Info
Section: Research Articles
Language : EN
Statistics:
  1. Collaboration Global Burden of Disease Cancer, Global, Regional, and National Cancer Incidence, Mortality, Years of Life Lost, Years Lived With Disability, and Disability-Adjusted Life-years for 32 Cancer Groups, 1990 to 2015: A Systematic Analysis for the Global Burden of Disease Study, JAMA Oncology, 3, 4, (2017) 524-548 https://doi.org/10.1001/jamaoncol.2016.5688
  2. Sandopu Sravan Kumar, Prabhakaran Manoj, Girish Nimisha and Parvatam Giridhar, Phytoconstituents and stability of betalains in fruit extracts of Malabar spinach (Basella rubra L.), Journal of Food Science and Technology, 53, 11, (2016) 4014-4022 https://doi.org/10.1007/s13197-016-2404-8
  3. Manju Singh, Rajesh Kumari, Durgesh Nandini and Mita Kotecha, Preliminary phytochemical screening of Basella rubra Linn, Journal of Pharmacognosy and Phytochemistry, 5, 4, (2016) 224
  4. Bhanu Priya Kilari, Venkata Subbaiah Kotakadi and Josthna Penchalaneni, Anti-proliferative and Apoptotic Effects of Basella rubra (L.) Against 1, 2-Dimethyl Hydrazine-induced Colon Carcinogenesis in Rats, Asian Pacific Journal of Cancer Prevention, 17, 1, (2016) 73-80 http://dx.doi.org/10.7314/APJCP.2016.17.1.73
  5. Azis Saifudin, Senyawa Alam Metabolit Sekunder: Teori, Konsep, dan Teknik Pemurnian, Deepublish, Yogyakarta, 2014
  6. P.D. Padmasari, K.W. Astuti and N.K. Warditiani, Skrining Fitokimia Ekstrak Etanol 70% Rimpang Bangle (Zingiber Purpureum Roxb.), Jurnal Farmasi Udayana, 2, 4, (2013) 1-7
  7. Cancer Chemoprevention Research Center, Prosedur Tetap Uji Sitotoksik Metode MTT, in, Fakultas Farmasi, Universitas Gadjah Mada, Yogyakarta, 2012, pp. 1-8
  8. Poppy Anjelisa Z. Hasibuan, Rosidah, Syafruddin Ilyas and M. Pandapotan Nasution, Efek Antiproliferatif Ekstrak N-Heksan Daun Bangun-Bangun (Plectranthus amboinicus, (Lour.) Spreng.) terhadap Sel MCF7, Seminar Nasional Biologi, Medan, (2013)
  9. Krishana Priya, Ankur Gupta, Surabhi Mahajan, RK Agnihotri and Rajendra Sharma, Evaluation of antimicrobial properties of Basella rubra methanolic extracts on selected microorganisms, International Journal of Pharmaceutical Sciences and Research, 6, (2015) 334-336
  10. Natthida Weerapreeyakul, Apiyada Nonpunya, Sahapat Barusrux, Thaweesak Thitimetharoch and Bungorn Sripanidkulchai, Evaluation of the anticancer potential of six herbs against a hepatoma cell line, Chinese Medicine, 7, 1, (2012) 15 https://doi.org/10.1186/1749-8546-7-15
  11. Yuliana Manik, Enny Fachriyah and Dewi Kusrini, Isolasi, Identifikasi dan Uji Sitotoksik Senyawa Steroid dari Daun Binahong (Anredera Cordifilia (Ten.) Stennis), Jurnal Kimia Sains dan Aplikasi, 16, 1, (2013) 23-26 https://doi.org/10.14710/jksa.16.1.23-26
  12. Ardji, Ari Widiyantoro and Lia Destiarti, Karakterisasi Senyawa Steroid dari Fraksi Diklorometana Batang Tanaman Andong (Cordyline fruticosa) dan Aktivitas Sitotoksiknya terhadap Sel HeLa, Jurnal Kimia Khatulistiwa, 7, 1, (2018) 48-52
  13. Ois Nurcahyanti, Euis Julaeha and Tri Mayanti, senyawa steroid dari kulit batang Dysoxylum alliaceum dan aktivitasnya terhadap sel kanker payudara MCF-7, Chimica et Natura Acta, 3, 2, (2015) 62-65 https://doi.org/10.24198/cna.v3.n2.9184
  14. Young-Sang Kim, Xi-Feng Li, Kyong-Hwa Kang, BoMi Ryu and Se Kwon Kim, Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells, BMB Rep., 47, 8, (2014) 433-438
  15. Sri Murni Astuti, Mimi AM Sakinah, Retno BM Andayani and Awalludin Risch, Determination of saponin compound from Anredera cordifolia (Ten) Steenis plant (binahong) to potential treatment for several diseases, Journal of Agricultural Science, 3, 4, (2011) 224 http://dx.doi.org/10.5539/jas.v3n4p224
  16. Irma Podolak, Agnieszka Galanty and Danuta Sobolewska, Saponins as cytotoxic agents: a review, Phytochemistry Reviews, 9, 3, (2010) 425-474 https://doi.org/10.1007/s11101-010-9183-z
  17. Puspita Arum Wijayanti, Ayik Rosita Puspaningtyas and Dian Agung Pangaribowo, Uji Sitotoksisitas dan Proliferasi Senyawa 1-(4- Trifluorometil benzoiloksimetil)-5-Fluorourasil terhadap Sel Kanker Payudara MCF-7 (Cytotoxicity and Proliferation Assay of 1-(4- Trifluoromethyl benzoyloxymethyl)-5-Fluorouracil) On MCF-7 Breast Cancer Cell, e-Journal Pustaka Kesehatan, 3, 3, (2015) 419-423
  18. Ardy Prian Nirwana, Aktivitas Antiproliferasi Ekstrak Etanol Daun Benalu Kersen (Dendrophtoe pentandra L. Miq.) terhadap Kultur Sel Kanker Nasofaring (Raji Cell Line), Biosain, Universitas Sebelas Maret, Surakarta
  19. Xiao-Huang Xu, Ting Li, M. Chi Fong, Xiuping Chen, Xiao-Jia Chen, Yi-Tao Wang, Ming-Qing Huang and Jin-Jian Lu, Saponins from Chinese Medicines as Anticancer Agents, Molecules, 21, 10, (2016) https://doi.org/10.3390/molecules21101326
  20. Rollando Rollando and Prilianti Kestrilia Rega, Fraksi Etil Asetat Kulit Batang Faloak (Sterculia Quadrifida R.br ) Menginduksi Apoptosis dan Siklus Sel pada Sel Kanker Payudara T47d, Journal of Pharmaceutical Sciences and Community, 14, 1, (2017) 1-14 http://dx.doi.org/10.24071/jpsc.141557
  21. Putri Y Suyanto, Ahmad R Utomo and Ferry Sandra, Mutasi Gen p53: Faktor Prediktif Kanker Payudara?, Indonesian Journal of Cancer, 2, 4, (2008) 138-143

Last update:

No citation recorded.

Last update:

No citation recorded.