Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D

Masayu Farah Diba; Salni Salni; Subandrate Subandrate

doi:10.14710/jksa.22.3.73-78

DOI: https://doi.org/10.14710/jksa.22.3.73-78

Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D

Cytotoxic Test of Extract and Fraction of Dendrophtoe Pentandra (L) Miq on T47D Cell

Masayu Farah Diba¹, Salni Salni²

, Subandrate Subandrate³

¹Biomedical Program, Faculty of Medicine, Universitas Sriwijaya, Indonesia

²Department of Biology, Faculty Mathematics and Natural Sciences, Universitas Sriwijaya, Indonesia

³Department of Biochemistry, Faculty of Medicine, Universitas Sriwijaya, Indonesia

Received: 14 Jan 2019; Revised: 8 Apr 2019; Accepted: 24 Apr 2019; Available online: 27 May 2019; Published: 31 May 2019.

BibTex Citation Data :

@article{JKSA22075,
    author = {Masayu Diba and Salni Salni and Subandrate Subandrate},
    title = {Uji Sitotoksik Ekstrak dan Fraksi Dendrophtoe pentandra (L) Miq pada sel T47D},
    journal = {Jurnal Kimia Sains dan Aplikasi},
  volume = {22},
    number = {3},
    year = {2019},
    keywords = {Cytotoxic; Dendrophtoe petandra (L.) Miq; Extract; Fraction},
    abstract = {Indonesia has a variety of plants that have the potential for medicine. One of the plants used by the community as a drug with anticancer effects is  Dendrophtoe petandra  (L.) Miq. This study aims to determine the cytotoxic effect of ethanol extract, ethyl acetate fraction and water ethanol fraction of  Dendrophtoe petandra  (L.) Miq on T47D breast cancer cells. The cytotoxic effects of ethanol extract, ethyl acetate fraction and water ethanol fraction were carried out by MTT assay method using series concentration. Cytotoxic effects were assessed by calculating IC 50  values using linear equations. Phytochemical test showed that  Dendrophtoe petandra  (L.) Miq contained saponins, terpenoids, flavonoids and tannins. The IC 50  value of the ethanol extract, ethyl acetate fraction and water ethanol fraction were 417.506 μg/mL, 233.617 μg/mL, and 2748.357 μg/mL, respectively. The smaller the IC 50  value means that the compound is more active. Water ethanol fraction of  Dendrophtoe petandra  (L.) Miq does not have a cytotoxic effect, whereas ethanol extract and ethyl acetate fraction of  Dendrophtoe petandra  (L.) Miq have cytotoxic effects in the medium strength category. The content of flavonoids and saponins in ethanol extract and ethyl acetate fraction of  Dendrophtoe petandra  (L.) Miq is thought to play role in causing T47D cell death. Cytotoxic effects of ethyl acetate fraction are stronger than ethanol extracts.},
   issn = {2597-9914},   pages = {73--78}  doi = {10.14710/jksa.22.3.73-78},
    url = {https://ejournal.undip.ac.id/index.php/ksa/article/view/22075}
}

Citation Format:

Abstract

Indonesia has a variety of plants that have the potential for medicine. One of the plants used by the community as a drug with anticancer effects is Dendrophtoe petandra (L.) Miq. This study aims to determine the cytotoxic effect of ethanol extract, ethyl acetate fraction and water ethanol fraction of Dendrophtoe petandra (L.) Miq on T47D breast cancer cells. The cytotoxic effects of ethanol extract, ethyl acetate fraction and water ethanol fraction were carried out by MTT assay method using series concentration. Cytotoxic effects were assessed by calculating IC₅₀ values using linear equations. Phytochemical test showed that Dendrophtoe petandra (L.) Miq contained saponins, terpenoids, flavonoids and tannins. The IC₅₀ value of the ethanol extract, ethyl acetate fraction and water ethanol fraction were 417.506 μg/mL, 233.617 μg/mL, and 2748.357 μg/mL, respectively. The smaller the IC₅₀value means that the compound is more active. Water ethanol fraction of Dendrophtoe petandra (L.) Miq does not have a cytotoxic effect, whereas ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq have cytotoxic effects in the medium strength category. The content of flavonoids and saponins in ethanol extract and ethyl acetate fraction of Dendrophtoe petandra (L.) Miq is thought to play role in causing T47D cell death. Cytotoxic effects of ethyl acetate fraction are stronger than ethanol extracts.

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Keywords: Cytotoxic; Dendrophtoe petandra (L.) Miq; Extract; Fraction

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In Vol 22, No 3 (2019): Volume 22 Issue 3 Year 2019

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