AKTIVITAS PREBIOTIK TEPUNG PISANG KEPOK (MUSA PARADISIACA FORMATYPICA) TERHADAP LACTOBACILLUS PLANTARUM DALAM MENGHAMBAT PERTUMBUHAN ESCHERICHIA COLI

PERMATASARI, DIAH AYU and Miksusanti, Miksusanti and Apriani, Elsa Fitria (2023) AKTIVITAS PREBIOTIK TEPUNG PISANG KEPOK (MUSA PARADISIACA FORMATYPICA) TERHADAP LACTOBACILLUS PLANTARUM DALAM MENGHAMBAT PERTUMBUHAN ESCHERICHIA COLI. Undergraduate thesis, Sriwijaya University.

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Abstract

Kepok banana (Musa paradisiaca formatypica) contains inulin and fructooligosaccharide (FOS) which act as natural prebiotics. This research was conducted to analyze the effect of kepok banana flour before and after modification with high pressure heating on the growth of L. plantarum using Design Expert 12®. Test standardization of the quality of banana flour kepok based on SNI 01-3751-2009. Modification of kepok banana flour with variations of TPM1, TPM2, TPM3, TPM4 with variations in temperature and heating time of 110˚C and 121˚C for 15 and 30 minutes. Resistance starch levels were determined using a UV-VIS spectrophotometer. Viability test of L. plantarum and test of prebiotic activity in inhibiting the growth of E. coli were carried out using the total plate count method. The kepok banana flour used has met the quality standards of SNI 01-3751-2009. With normal organoleptic, fineness 99.05%, moisture content 8.9% and ash content 0.5%. The resistant starch content of kepok banana flour before and after modification (optimum conditions) was 23.208% ± 5.115 and 38.529% ± 1.238. The viability test showed that the results of kepok banana flour before modification stimulated the highest growth of L. plantarum with an increase percentage of 7.658%. The prebiotic index value of kepok banana flour before and after modification was 1.193 and 1.119. The prebiotic activity value of kepok banana flour before and after modification was 1.319 and 0.844. The optimum condition of modified flour was obtained at 110˚C for 30 minutes. The positive value of prebiotic activity indicates that the two media are effective as prebiotic candidates and can reduce the number of E. coli growth.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Tepung Pisang kepok, Prebiotik, L. plantarum, E. coli
Subjects:	R Medicine > RS Pharmacy and materia medica > RS160-167 Pharmacognosy. Pharmaceutical substances (Plant, animal, and inorganic)
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Diah Ayu Permatasari
Date Deposited:	31 Mar 2023 03:40
Last Modified:	31 Mar 2023 03:40
URI:	http://repository.unsri.ac.id/id/eprint/92091

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