PENGARUH PEMBERIAN SOLID LIPID NANOPARTICLE PEMBAWA GLIBENKLAMID TERHADAP KADAR GLUKOSA DARAH TIKUS (Rattus norvegicus) JANTAN MENGGUNAKAN METODE TOLERANSI GLUKOSA

LUFIANTI, AFIFAH and Herlina, Herlina and Mardiyanto, Mardiyanto (2018) PENGARUH PEMBERIAN SOLID LIPID NANOPARTICLE PEMBAWA GLIBENKLAMID TERHADAP KADAR GLUKOSA DARAH TIKUS (Rattus norvegicus) JANTAN MENGGUNAKAN METODE TOLERANSI GLUKOSA. Undergraduate thesis, Sriwijaya University.

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Abstract

A study of the effect of glibenclamide loaded solid lipid nanoparticle (SLN) on decreased blood glucose levels was imposed on normal rats. Glibenclamide loaded SLN production of 3 batches with each EE percentage of 83.023 ± 1.725%, 81.536 ± 0.664%; and 81.903 ± 0.953%. The resulting glibenclamide loaded SLN preparation has an average particle size of 67.1 nm, PDI 0.061, and zeta potential -1.4 mV. Glibenclamide loaded SLN had a greater diffussion percentage at 720 minutes than tablet of glibenclamide on the market and glibenclamide pure powder, 36,46 ± 0,535%, 33,563 ± 0,271%, dan 24,140 ± 0,146%, respectively. This study used the glucose tolerance test, by way of burdening the normal rats that have been fasted for 20 hours with glucose. Body weight of rats used 150 – 250 g, age 2 – 3 months. Rats were divided into five groups, including negative control group (Na CMC 1%), positive control group (tablet of glibenklamid dose 1.89 mg/kgBB), glibenclamide loaded SLN group with three dosage ratings 0.95; 1.89; and 3.78 mg/kgBB. Giving glucose at loading is 6.75 g/kgBB. Blood was taken from the lateral veins of the tail at minute 0, 30, 60, 90, 120, 150, and 180. Blood glucose levels determined using a EasyTouch® glucometer. AUC0-180 of each group were 26043 ± 33.63 (negative control), 17340 ± 10.39 (positive control), 16587 ± 11.73 (dose 1), 15618 ± 12.96 (dose 2), and 14700 ± 17.00 (dose 3). Percent reduction in blood glucose levels obtained by positive control with dose of 1.89 mg/kgBB in the amount of 50,190%, while glibenclamide loaded SLN at a dose of 0.95; 1.89; and 3.78 mg/kgBB showed percent reduction in blood glucose levels of 57.008%; 66.749%; and 77.163%. Based on statistical test using one way ANOVA with 95% confidence level, there were no significant differences between group of positive control with group of dose 1, dose 2, and dose 3 (p>0.05).

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: solid lipid nanoparticle, glibenclamide, diabetes mellitus, glucose tolerance method, blood glucose levels
Subjects: Q Science > QD Chemistry
R Medicine > RS Pharmacy and materia medica
Divisions: 08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User: Mrs Dies Meirita Sari
Date Deposited: 11 Jul 2019 03:39
Last Modified: 18 Jul 2019 15:14
URI: http://repository.unsri.ac.id/id/eprint/113

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