UJI AKTIVITAS ANTIBAKTERI FRAKSI DAN SENYAWA AKTIF DAUN GELAM (Melaleuca cajuputi Powell) TERHADAP Escherichia coli DAN Staphylococus aureus

SETIANI, LILIS and Salni, Salni and Marisa, Hanifa (2017) UJI AKTIVITAS ANTIBAKTERI FRAKSI DAN SENYAWA AKTIF DAUN GELAM (Melaleuca cajuputi Powell) TERHADAP Escherichia coli DAN Staphylococus aureus. Undergraduate thesis, Sriwijaya University.

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Abstract

Infectious disease is one of the most dangerous diseases in Indonesia. Some types bacteria that can cause infectious diseases include E. coli and S. aureus. Infectious can be overcome antibacterial with a certain concentration. Objective study antibacterial activity of fraction and active compound gelam leaves to E. coli and S. aureus is find and determine the type of active fraction, a group active compounds from gelam leaves that can be used as antibacterial to E. coli and S. aureus, find and determine minimum inhibitory concentration (MIC) fraction and active compound of gelam leaves to E. coli and S. aureus. Antibacterial activity of fraction and active compound of gelam leaves to E. coli and S. aureus was conducted on October 2016 until December 2016. The research method used was exploratory and experimental method. Exploration method was sampling of gelam leaves while the experimental method was activity test fraction gelam leaves, determination of MIC active fraction of leaf salt, bioautografi test and determination active compound class and determination MIC active compound leaf gelam. Results showed that the methanol-water fraction is the most active fraction compared to the fraction of n-hexane and ethyl acetate to E. coli and S. aureus. Active compounds of leaf salt on thin layer chromatography (TLC) and bioautography testing is indicated as a tannin compound characterized by brown spots with Rf 0.25. The minimum inhibitory concentration methanol-water fraction on E. coli ml and S. aureus . Minimum inhibitory concentration isolates M1 against E. coli S. aureus was 125 l.It can be concluded that active fraction of testing antibacterial activity gelam leaves to E. coli and S. aureus was the methanol-water fraction and good fraction. Secondary metabolite contained leaves was active compound of tannins classified by brown spots Rf 0.25. Leaves were potentially antibacterial with a minimum inhibitory concentration (MIC) water-le ml against E. coli and 25 S. aureus, where as MIC testing of tannin compounds against E. coli /ml, while against S. aureus

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Leaves Gelam (Melaleuca cajuputi Powell.), Escherichia coli, Staphylococcus aureus, Antibacterial Activity Test
Subjects: Q Science > QH Natural history > QH301 Biology
Divisions: 08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User: Mrs Dies Meirita Sari
Date Deposited: 26 Nov 2019 03:11
Last Modified: 26 Nov 2019 03:11
URI: http://repository.unsri.ac.id/id/eprint/18385

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