PEMANFAATAN MADU SEBAGAI EKSTENDER UNTUK KRIOPRESERVASI SPERMA IKAN GABUS (CHANNA STRIATA)

MANGKUNEGARA, ANUGERAH AL AMIN and Dwinanti, Sefti Heza and Syaifudin, Mochamad (2019) PEMANFAATAN MADU SEBAGAI EKSTENDER UNTUK KRIOPRESERVASI SPERMA IKAN GABUS (CHANNA STRIATA). Undergraduate thesis, Sriwijaya University.

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Abstract

Cryopreservation of stripped snakehead is needed to improve reproduction quality of seeds and broodstock. Some components are important for cryopreservation including quality and quantity of extender. The aim of this study was to investigate the influence honey as extender at different ratio on channa sperm cryopreservation quality. The research used response surface methodology (RSM) with three treatments (T); 300 mg. ml-1 (T1), 400 mg. ml-1 (T2) and 500 mg. ml-1 (T3), which all treatments was replicated 3 times. The processed semen was equilibrated at 5°C for 3 h (chilling), packed into straws (0.25 ml), frozen and stored in a liquid nitrogen tank for 24 h before thawing and assessment of quality. Parameters observed were sperm characteristics such as (color, pH, concentration and sperm volume), motility and viability before and after cryopreservation. The results of this study indicated best treatment was honey concentration at 400 mg.ml-1 (T2). After 7 days preservation, the motility value was about 40 – 70 % (4 score), viability percentage was 64.15% and no abnormality was occurred on stripped snakehead. Keywords: Stripped snakehead sperm, cryopreservation, honey

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Kriopreservasi, madu, sperma ikan gabus.
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling
S Agriculture > SH Aquaculture. Fisheries. Angling > SH151-179 Fish culture
S Agriculture > SH Aquaculture. Fisheries. Angling > SH151-179 Fish culture > SH155.6.M48 Fishes--Artificial spawning. Fishes--Germplasm resources--Cryopreservation. Shellfish--Artificial spawning.
Divisions: Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User: Anugerah Al-Amin Mangkunegara
Date Deposited: 02 Aug 2019 10:26
Last Modified: 05 Oct 2019 14:57
URI: http://repository.unsri.ac.id/id/eprint/1966

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