DNA Authentication of Asian Redtail Catfish Hemibagrus nemurus from Musi and Penukal River, South Sumatra Indonesia

Mochamad, Syaifudin and Dade, Jubaedah and Muslim, Muslim and Ayu, Daryani (2017) DNA Authentication of Asian Redtail Catfish Hemibagrus nemurus from Musi and Penukal River, South Sumatra Indonesia. Genetics of Aquatic Organisms, 1 (.). pp. 43-48. ISSN 2459-1831

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Abstract

Asian Redtail Catfish (Hemibagrus nemurus) is highly economic important catfish in South Sumatra. Investigating DNA authentication is of importance for species conservation and breeding selection. Cytochrome C Oxidase subunit I is one of mitochondrial DNA markers used for species barcoding in freshwater, brackishwater and marine fish. This research aims to explore the use of COI gene for species barcoding, constructing phylogenetic tree of Asian Redtail Catfish (ARC). The methods used in the research consisted of DNA extraction, PCR (Polymerase Chain Reaction) amplification and sequencing mtDNA COI gene of ARC obtained from Musi and Penukal Rivers (South Sumatra). A 572 and 596 base pairs of partial coding sequences were obtained representing each river. Phylogenetic analyses indicated that ARC was at the same cluster from Bagridae family, and different cluster from others catfish, Pangasius sp and Oreochomis niloticus. Further study using more species of Bagridae and habitat are needed to investigate the diversity of DNA from South Sumatra water resources

Item Type: Article
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling
Divisions: 05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User: Muslim Muslim
Date Deposited: 07 Jan 2020 02:29
Last Modified: 21 Jan 2020 09:21
URI: http://repository.unsri.ac.id/id/eprint/23123

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