Melki, Melki and Mustopa, A Zaenal and Effendi, Hefni
(2011)
Bio-potentials Activity of Sonneratia caseolaris (Mangrove) Extract as Antibacterial Collected from The South Sumatera.
In: The International Symposium on Marine Ecosystems and the issue of Marine Natural Products and their Bioactive Metabolits, 25-27 October 2011, Bogor.
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Text (Bio-potentials Activity of Sonneratia caseolaris (Mangrove) Extract as Antibacterial Collected from The South Sumatera)
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Abstract
Crude extracts of four mangrove species (leaf, fruit, bark and root), i.e. Avicennia alba, A. marina, Rhizophora mucronata, and Sonneratia caseolaris collected from Teluk Payo, Banyuasin, South Sumatera was extracted in methanol, ethyl acetate, n-hexane and tested for different range of biological activities including antimicrobial activity isolates of Escherichia coli and Staphylococcus aureus pathogen and brine shrimp cytotoxic. The highest activity was recorded with the methanol extract of S. caseolaris in E. coli isolates (18 mm) and in S. aureus isolates (19 mm) exhibited more biopotency. Brine Shrimp Lethality Test showed that leaf of S. caseolaris methanolic was not toxic to Artemia salina. The highly active mangrove was evaluated further to analyze the chemical compounds using column chromatography with chloroform:methanol (9:1 to 1:9) eluted gave the best separation, thin layer chromatography with chloroform:methanol (9:1 to 1:9) eluted gave the best separation, and high performance liquid chromatography with detector photodiode array (PDA) at a wavelength of 200-400 nm showed mangrove extracts including flavonoid which might have functional role in bioactivity and can be used for the development of pisicides and biopharmaceuticals.
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