Hermansyah, Hermansyah (2012) Yeast Saccharomyces cerevisiae as model to identify mengkudu (morinda citrifolia) as an anticancer medicinal plants candidates with antiproliferative properties (Similirity). Unsri, Unsri. (Unpublished)
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Abstract
Although yeast Saccharomyces cerevisiae is an excellent eukaryotic model organism, however, to use S.cerevisiae cell as a model organism screening drugs is rare. In this research, we used S. cerevisiae to screen antiproliferative properties of mengkudu or noni fruit (Morinda citrifolia). The results showed that 3% w/v extract of mengkudu, but not pare and sukun extracts was capable to inhibit growth of S. cerevisiae effectively. This inhibited phenotypes was confirmed by measuring growth rate of cells in cultures treated with various concentrastions of extracts. Morphologycal analysis using differential interference contrast (DIC) microscopy showed that S. cerevisiae wild type strain FY833 cells was accumulated in the unbudded cells, and further analysis using Fluorescence Activated Cell Sorting (FACS) analysis exhibited that proliferation of cells run unproperly because of cells are arrested in G1 phase, indicating that G1 to S phase transition progression of S. cerevisiae cell cycle was inhibited in the presence of mengkudu extract.
| Item Type: | Other |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD415-436 Biochemistry |
| Divisions: | 08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1) |
| Depositing User: | Hermansyah Hermansyah |
| Date Deposited: | 29 Aug 2020 12:43 |
| Last Modified: | 31 Aug 2020 06:58 |
| URI: | http://repository.unsri.ac.id/id/eprint/34107 |
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