AKTIVITAS ANTIDIARE KOMBINASI FRAKSI N-HEKSANA DAN ETIL ASETAT DAUN KARAMUNTING (Rhodomyrtus tomentosa (Ait.) Hassk.) PADA TIKUS PUTIH JANTAN GALUR WISTAR TERINDUKSI BAKTERI Shigella dysentriae

MUKHLIFAH, ATIK PUPUT and Salni, Salni and Herlina, Herlina (2020) AKTIVITAS ANTIDIARE KOMBINASI FRAKSI N-HEKSANA DAN ETIL ASETAT DAUN KARAMUNTING (Rhodomyrtus tomentosa (Ait.) Hassk.) PADA TIKUS PUTIH JANTAN GALUR WISTAR TERINDUKSI BAKTERI Shigella dysentriae. Undergraduate thesis, Sriwijaya University.

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Abstract

Karamunting (Rhodomyrtus tomentosa (Ait.) Hassk) is traditional medicine has been used to treat several types of diseases caused by bacterial infections, such as diarrhea-dysentery. Diarrhea-dysentery is still common in developing countries. This disease is caused by a bacterium called Shigella dysenteriae. This study aims to evaluate the combination of n-hexane and ethyl acetate fractions from karamunting leaves as antibacterial. This research was conducted by in vivo method using male white rats (Rattus norvegicus) Wistar Strain induced by Shigella dysentriae. The treatment group was divided into five groups: negative control group, positive control group, and three treatment groups with doses 25, 50, and 100 mg/kg BW. Antibacterial activity test was carried out for 12 days by inoculating rat feces that has been diluted into SSA media. Negative controls using Na-CMC and ciprofloxacin were used as positive control. The extraction was carried out using a soxhlet gradually, starting with n-hexane then ethyl acetate. The results showed that a dose of 50 mg/kg BW was able to reduce the number of bacterial colonies on the 10th day with total of colonies 19.6 x 105 (cfu g-1) and a dose of 100 mg/kg BW on the 8th day with total of colonies 11.4 x 105 (cfu g-1). The combination of n-hexane and ethyl acetate fractions with a dose of 100 mg/kg BW can reduce the population of Shigella dysenteriae to be zero after 12 days of treatment. The ability to reduce the number of bacterial colonies from the combination of n-hexane and ethyl acetate fractions at a dose of 100 mg/kg BW is the same as the positive control ability of ciprofloxacin. Based on Duncan's statistical test results it is known that the treatment of a dose of 100 mg/kg BW and ciprofloxacin as a positive control showed an equally low bacterial population. Characterization of the ethyl acetate fraction of karamunting leaves obtained water-soluble extract content of 53.33%, ethanol soluble extract content 73.33%, moisture content 8.3%, drying loss 8.7 %, total ash content 1%, acid insoluble ash content 0.62%, microbial contamination test <10 cfu/g* (*EAPC), Cd metal contamination test 0.1658 mg/kg, and contamination test metal Pb 10.8130 mg/kg. The test for Pb metal contamination of ethyl acetate fraction did not meet the requirements. Karamunting leaves have the potential to be developed into a dysentery medicine.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Karamunting, kombinasi fraksi n-heksana dan etil asetat, antibakteri, S. dysenteriae
Subjects: Q Science > QR Microbiology > QR75-99.5 Bacteria
R Medicine > RM Therapeutics. Pharmacology > RM1-950 Therapeutics. Pharmacology
R Medicine > RS Pharmacy and materia medica > RS200-201 Pharmaceutical dosage forms
Divisions: Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User: Atik Puput Mukhlifah
Date Deposited: 05 Oct 2020 04:37
Last Modified: 05 Oct 2020 04:46
URI: http://repository.unsri.ac.id/id/eprint/36143

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