Short Communication: DNA barcodesand phylogenetic of striped snakehead and ocellated snakehead fish from South Sumatra, Indonesia

Syaifudin, Mochamad and Wijayanti, Marini and Dwinanti, Sefti Heza and Muslim, Muslim and Mahendara, Muhammad and Marliana, Shely (2020) Short Communication: DNA barcodesand phylogenetic of striped snakehead and ocellated snakehead fish from South Sumatra, Indonesia. Biodiversitas (Journal of Bological Diversity), 21 (3). pp. 1227-1235. ISSN 2085-4722

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Abstract

Abstract. Syaifudin M, Wijayanti M, Dwinanti SH, Muslim, Mahendra M, Marliana S. 2020. DNA barcodes and phylogenetic of striped snakehead and ocellated snakehead fish from South Sumatra, Indonesia.Biodiversitas 21:1227-1235.This research aimed to identify the sequencesof cytochrome c oxidase subunit I gene mitochondrialDNA (COI mtDNA), to construct aphylogenetic tree of striped snakehead (Channa striata) and ocellated snakehead (Channa pleuropthalma), and to measure water quality of Kelekar River, Indralaya, Ogan Ilir Districtand Danau Burung Besar River, Penukal Abab Lematang Ilir (PALI)Districtin South Sumatra,Indonesia. The research procedures consisted of DNA isolation, amplification by PCR (Polymerase Chain Reaction) and sequencing of fragmentCOI mtDNA. The length of nucleotide was 604bp for striped snakehead and 587-604 bp for ocellated snakehead. Optimum annealing temperature was 500C for 15 seconds with 30 cycles. The result of BLAST analysis showed that striped snakehead from Kelekar and Danau Burung Besar River had 100% identity to striped snakehead from Java-Bali and furthest (97%) with striped snakehead from India. Ocellated snakehead had 100% similarity with the same species from Musi Banyuasin and Banjarmasin; and furthest (83%) with Channa limbata from Myanmar. Water quality in Kelekar River were temperature 31-31.60C, pH 4.76-4.96, dissolved oxygen 2.7-3.0 mg/L, ammonia <0.009 mg/L, total alkalinity 20 mg/L, and turbidity62.5-63 cm. Meanwhile in Danau Burung Besar River showed temperature (29.3-30.70C), pH (3.6-6.7), dissolved oxygen (1.31-3.76 mg/L), ammonia (0.17-0.20 mg/L), and turbidity(50-90 cm

Item Type: Article
Uncontrolled Keywords: COI, ocellated snakehead, phylogenetics, striped snakehead
Subjects: S Agriculture > SH Aquaculture. Fisheries. Angling > SH20.3-191 Aquaculture
Divisions: 05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User: Muslim Muslim
Date Deposited: 24 Jan 2021 16:31
Last Modified: 24 Jan 2021 16:31
URI: http://repository.unsri.ac.id/id/eprint/40914

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