EFEKTIVITAS BACILLUS THURINGIENSIS SEBAGAI BIOINSEKTISIDA PADA TANAMAN JAGUNG (ZEA MAYS L.) TERHADAP SERANGAN ULAT GRAYAK (SPODOPTERA FRUGIPERDA)

INDRIANI, RISKHA and Pujiastuti, Yulia (2020) EFEKTIVITAS BACILLUS THURINGIENSIS SEBAGAI BIOINSEKTISIDA PADA TANAMAN JAGUNG (ZEA MAYS L.) TERHADAP SERANGAN ULAT GRAYAK (SPODOPTERA FRUGIPERDA). Undergraduate thesis, Sriwijaya University.

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Abstract

Fall armyworm (Spodoptera frugiperda) are introduced pests that have been infesting maize crops in Indonesia since 2019. The armyworm feed on young leaves causing hollow on the leave and disturbs the growing tip of the plant. Various control methods have been applied by farmers, among others is by using commercial insecticide. In this research, B. thuringiensis isolate were supplied by laboratory of Phytopathology, Department of Plant Pest and Disease, Sriwijaya University. The experiment was arranged in a Randomized Block Design (RBD) with 6 treatments and 5 replications. The treatments were control, P1 (5 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker), P2 (10 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker), P3 (15 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker), P4 (20 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker), P5 (1 g Dipel WG + 1 L water + 1 ml Nasa sticker). The insect used in the research was second instar of S. frugiperda for which 25 larvae were treated in each replication. Parameters observed included spore density, plant eight, damage symptoms caused by the insect on maize plant, larval mortality, symptom on dead larvae, larval death examination, daily temperature, and daily relative humidity in the maize cultivation. Data of plant height and larval mortality were analyzed using ANOVA, while data of spore density, damage on plant, larval mortality, larval death examination, temperature and humidity were analyzed descriptively. Spore was counted at 24, 48 and 72 hours after preparation. Spore density at 72 hours was 4,38 x 1012 spores/ml. Application of B. thuringiensis wa conducted when maize plant was 4 weeks old (after larval infestation). The results showed that plant height differed among treatments started at fifth week. At the fifth to sixth weeks, the highest plant was found in treatment P3 (15 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker), while at seventh week, the highest plant was found in treatment P4 (20 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker). The damage symptom on maize plant caused by fall armyworm included feeding trace on leaf, larval feces remains, and hollow on folded leaves. The larval death symptom caused by B. thuringiensis were the blackish brown color, softened body, liquid leaking, and sting flavor. To identify the causal agent of larval death, larval death examination was made and the result convinced that the larvae were infected by Bacillus thuringiensis. The results of the research showed that P4 (20 ml B. thuringiensis + 1 L water + 1 ml Nasa sticker) was the best treatment causing larval mortality up to 88% at 36 hours and supportive to maize plant growth.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Spodoptera frugiperda, Bacillus thuringiensis, bioinsektisida.
Subjects: S Agriculture > S Agriculture (General) > S539.5-542.3 Research. Experimentation
Divisions: 05-Faculty of Agriculture > 54295-Plant Protection (S1)
Depositing User: Riskha Indriani
Date Deposited: 27 Jan 2021 02:11
Last Modified: 27 Jan 2021 02:11
URI: http://repository.unsri.ac.id/id/eprint/41132

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