VARIASI GENETIK DUKU DAN LANGSAT (Lansium domesticum Corr.) DI SUMATERA SELATAN BERDASARKAN PADA DNA BARCODING matK

NURSELA, DEWI and Hanum, Laila and Muharni, Muharni (2021) VARIASI GENETIK DUKU DAN LANGSAT (Lansium domesticum Corr.) DI SUMATERA SELATAN BERDASARKAN PADA DNA BARCODING matK. Undergraduate thesis, Sriwijaya University.

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Abstract

Duku and Langsat (Lansium domesticum Corr.) are one of the typical plants of South Sumatera that have high economic value, so that their existence needs to be preserved, one of which is by carrying out plant breeding. Genetic identity and and clear taxonomic status to determine the next steps of conesrvation strategies. Therefore, in determining genetic identity it is necessary to condust research on genetic variation of Duku and Langsat (Lansium domesticum Corr.)in South Sumatera based on the nucleotide base sequence in the matK gene in an effort determine the taxonomic status of Duku and Langsat. This research aims to determine genetic variation of Duku and Langsat (Lansium domesticum Corr.)in South Sumatera based on the nucleotide base sequence in the matK gene in an effort determine the taxonomic status of Duku and Langsat. This research was conducted from February to November 2020 at the Laboratory of Genetics and Biotechnology, Departemen of Biology, Faculty of Mathematics and Natural Sciences, Sriwijaya University, Indralaya. The method of this research is DNA extraction using Tiangen DNA Plant Genomic KIT DP 305 the DNA amplification using Primers 3F_KIM-F dan 1R_KIM-R, electrophoresis and sequencing DNA. The result of this study showed that Duku and Langsat in South Sumatera Showed quiet high genetic variation as indicated by the value of identity percentage 97,41%-99,50% , and determined as Lansium domesticum Corr. based on matK gene marker. Keyword : Genetic variation, Duku, Langsat, Lansium domesticum, DNA Barcoding, matK genes.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: DNA BARCODING MATK, DUKU DAN LANGSAT
Subjects: Q Science > Q Science (General) > Q1-295 General
Divisions: 08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User: Users 10336 not found.
Date Deposited: 05 Mar 2021 04:07
Last Modified: 05 Mar 2021 04:07
URI: http://repository.unsri.ac.id/id/eprint/43496

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