TOKSISITAS INSEKTISIDA ORGANOFOSFAT TERHADAP MORTALITAS IKAN MAS (Cyprinus carpio L.)

SEFTIANI, NESA and Junaidi, Endri and Purwoko, Agus (2021) TOKSISITAS INSEKTISIDA ORGANOFOSFAT TERHADAP MORTALITAS IKAN MAS (Cyprinus carpio L.). Undergraduate thesis, Sriwijaya University.

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Abstract

Organophosphates is synthetic pesticides Organophosphates consists of phosphoric acid or thiophosphate esters which are acutely toxic to vertebrates such as fish, birds, lizards and mammals. One example of an organophosphate insecticide is Dursban 200EC with the active ingredient in the form of chlorpyrifos. Goldfish (carp) (Cyprino carpio L.) is a fresh water fish. The survival of goldfish isdepended on waters quality. Goldfish have a high level of sensitivity and are susceptible to pestisides pollution. Goldfish is bioassay reserarch test biota. Research objectives were to observe fish behavior on expossingorganophosphate insecticides, to determine the LC50 value of organophosphate insecticides and to determine the first concentration that has a real impact on carp, and to determine the concentration that begins to affect the life and behavior of fish. Research goals are to to provide information about the effect of organophosphate insecticides on non target animals. Nely had conducted research was an experimental method using a randomized block design and was experimental method using a randomized block design and was analyzed by using a static test (non-renewal). The experimental group was given several treatments within a certain period of time, after which observation and analysis of the dependent variable were carried out on the independent and control variables. Concentration organophosphate insecticides are: 0 ml / L act as (control) ; 0.25 ml / L; 0.75 ml / L; 1.25 ml / L and 1.75 ml / L and observed every 24 hours, 48 hours, 72 hours, and 96 hours. The data analysis used in this research is analysis of variance and further test of BNJ (LSD). The results showed that at the 24 hour exposure time the LC50 value was 0.944 ml/L, the 48 hour LC50 value was 0.681 ml/L, the 72 hour LC50 value was 0.095 ml/L, and the LC50 96 hours was 1.529 ml/L. The cause of fish mortality is exposure to organophosphate insecticides that contain toxic substances so that the fish find it difficult to adapt to their environment which causes changes i gills color from red to black and fishbecomed slimy. The TUa value obtained from exposure for 24, 48, 72 and 96 hours respectively was 1.059; 1,468; 10.52; 0.654. Based on the TUa value, the 24 hours and 48 hours organophosphate insecticides were classified as Significant Acute Toxicity, 72 hours High Acute Toxicity and 96 hours No Acute Toxicity. Based on the ANOVA test, it is known that the duration of exposure time has no effect on goldfish mortality (p>0.05), while the treatment of organophosphate insecticide concentrations on carp has a toxic effect resulting in significant (p<0.05) mortality of carp. The result of further test showed that the letter notation was different from the treatment. Group a is the control treatment, which is included in group b, namely the treatment with a concentration of 0.25 ml/L, 0.75 ml/L, and 1.25 ml/L. Then those who entered into group c were treatment with a concentration of 1.75 ml/L. Grouping based on the same letter notation indicates a significant value. The treatment which has a different letter notation means that the treatment has a significant difference. Table 4.9. shows that the higher the concentration given, the higher the mortality of goldfish. The results of statistical analysis using ANOVA showed that concentrations of 0.25 ml/L, 0.75 ml/L, 1.25 ml/L, 1.75 ml/L caused significant mortality of goldfish. Based on the calculation of LC50, the concentration of 0.944 ml/L caused the death of goldfish as much as 50% within 24 hours, the concentration of 0.681 ml/L caused the death of 50% of goldfish within 48 hours, a concentration of 0.095 ml/L caused the death of 50% of goldfish within 72 hours. hours, and a concentration of 1.529 ml/L caused 50% mortality within 96 hours. The conclusion are fish got stress and lose its balance, weak movement or swimming slimy cales, opening operculum pale gills drive fish to death. Lethal concentration (LC50) of organophosphate insecticide for 24 hours was 0.944 ml/L, 48 hours was 0.681 ml/L, 72 hours was 0.095 ml/L, and 96 hours was 1.529 ml/L. The first concentration that has a significant impact on carp (Cyprinus carpio L.) is 0.25 ml/L. The concentration that began to affect the mortality of carp (Cyprinus carpio L.) was 0.25 ml/L. The higher the concentration given, the higher the mortality of carp. This means that the use of organophosphate insecticides with a concentration of 2 ml/L is very toxic to non-target animals.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Dursban 200EC, Ikan mas (Cyprinus carpio L.), LC50, Organofosfat, Toksisitas.
Subjects: Q Science > QP Physiology
Divisions: 08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User: Nesa Seftiani
Date Deposited: 30 Jun 2021 04:20
Last Modified: 30 Jun 2021 04:20
URI: http://repository.unsri.ac.id/id/eprint/48835

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