ISOLASI METABOLIT SEKUNDER DARI JAMUR ENDOFITIK (Aspergitlus niger) PADA KULIT BATANG TUMBUHAN KANDIS GAJAH (Garcinia griffithii T.ANDERS) DAN UJI AKTIVITAS ANTIOKSIDANNYA

ARYANI, SEPTA and Elfita, Elfita and Muharni, Muharni (2012) ISOLASI METABOLIT SEKUNDER DARI JAMUR ENDOFITIK (Aspergitlus niger) PADA KULIT BATANG TUMBUHAN KANDIS GAJAH (Garcinia griffithii T.ANDERS) DAN UJI AKTIVITAS ANTIOKSIDANNYA. Undergraduate thesis, Sriwijaya University.

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Abstract

The secondary metabolites compound heve been isolated from endophytic fungi {Aspergillus niger) from stem bark of kandis gajah {Garcinia griffithii T. anders). Isolation begin with cultivation of Aspergillus niger fungi in 5 L of PDB’s media {Potato Dextrose Broth ) for eight weeks. Media i s extracted into the solvent ethyl acetate following by evaporation. Ethyl acetate extracts were separated by chromatography techniques to get pure compound in form of white crystal counted 71 mg. The H-NMR spectrum showed four proton aromatic that it signal showed at 8H 7.22; 7.27; 7.27; 7.20 proton sp2 was 8H 6.00 and5.56 and one proton sp3 methyne was SH 4,90 ppm. The 13C-NMR spectrum showed isolated compound have 18 carbons. Base on to analysis result using H-NMR, i3C-NMR, HMQC, HMBC, and COSY the resulting isolation compound spevtroscopy as 8,10,12 -trihydroxy- 9- methoxy -7a-methyl -7,7a,12a,13-tetrahydrobenzo cycloheptaoxocin-6-on with molecule formula CigH2o06- The isolation compound showed activity as antioxidant and has 1C50 value 97.24.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	endophytic fungi, Aspergillus niger, Garcinia griffithii
Subjects:	Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	01 Mar 2022 08:09
Last Modified:	01 Mar 2022 08:09
URI:	http://repository.unsri.ac.id/id/eprint/65494

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