ISOLASI B-SITOSTEROL DARI EKSTRAK ETIL ASETAT RUAH MAHKOTA DEWA (Phaleria macrocarpa L)

SEPTIANI, LELY and Muharni, Muharni and Elfita, Elfita (2013) ISOLASI B-SITOSTEROL DARI EKSTRAK ETIL ASETAT RUAH MAHKOTA DEWA (Phaleria macrocarpa L). Undergraduate thesis, Sriwijaya University.

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Abstract

Isolation of p-sitosterol from ethyl acetate exstract of mahkota dewa fruit (Phaleria macrocarpa L) had been done. Isolation was first done by maceration using increasing polarity (n-hexane and ethyl acetate). Separation and purification were done by chromatography technique. Structural determination of isolated compound was done by analysis data of NMR spectroscopy. The isolated compound is a white powder with melting point 135°-136°C. Spectrum of 'HNMR shows a signal for CH sp2 at 5.34 ppm which is characteristic for double bond at position C-5 and C-6 in steroid. Signal at 3.52 ppm (IH, m) is a signal specific for CH proton bonded to carbon atom attached to hydroxyl group at C-3 position. It is also observed a high intensity signal at 8h 0.68 ppm (3H, 5), 0.80-0.85 ppm (9H), 0.91 (3H, d) and 8h 1.00 ppm (3H, s) which are signal for protons for six methyl groups. Other signals that accumulate in the region under 8h 2.30 ppm are signals for proton sp3 in CH and CH2. Spectrum of 13C-NMR shows that there are 29 signals, and based on DEPT spectrum these signals consisted of 6 signals of primary carbon, 11 signals of secondary carbon (CH2), 9 signals of tertiary carbon (CH) and 3 signals of quatemary carbon. According to the analysis of spectroscopic data and comparison to literature data, it is concluded that the isolated compound is a steroid, that is P-sitosterol with molecular formula C29H50O.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Phaleria macrocarpa L, steroid, B-sitosterol.
Subjects:	Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	02 Mar 2022 01:36
Last Modified:	02 Mar 2022 01:36
URI:	http://repository.unsri.ac.id/id/eprint/65653

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