UJI TOKSISITAS DAN ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK METANOL BATANG TUMBUHAN KATEMAS (Euphorbia geniculata Ortega)

FATSIAMI, RICCE and Eliza, Eliza and Yusuf, Setiawati (2014) UJI TOKSISITAS DAN ISOLASI SENYAWA TRITERPENOID DARI EKSTRAK METANOL BATANG TUMBUHAN KATEMAS (Euphorbia geniculata Ortega). Undergraduate thesis, Sriwijaya University.

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Abstract

The aim of this research are isolated and characterized triterpenoid from methanol extract of katemas stem {Euphorbia geniculata ortega). To evaluate toxicity of methanol exctract have been conducted mortality test against armyworm larvae {spodoptera litura). The assay was carry out with no choise test method using variances of extract methanol concentration (0%, 1%, 2%, 3%, 4%, 5%). The highest mortality was displayed at 5% concentration while the 50% of lethal concentration (LC50) at 3,92%. The isolation process was conducted by a series work steps such as extraction, fractionation and purification. Extraction process was done by maceration method using methanol as a solvent. Fractionation and purification were conducted by a vary of chromatography techniques. Three compounds have been succesful isolated from isolation proces i.e. compound 1 (63 mg), compound 2 (1 mg), and compound 3 (2 mg). One of them (compound 1) has been identified by spectroscopy data analysis ('H-NMR, uC-NMR, HSQC and HMBC). The identified compound is pentacyclic triterpenoid namely lupeol asetat.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Euphorbia geniculata Ortega, Spodoptera litura, LC50, lupeol acetate.
Subjects: Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions: 08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User: Mrs Furika Furika
Date Deposited: 02 Mar 2022 01:40
Last Modified: 02 Mar 2022 01:40
URI: http://repository.unsri.ac.id/id/eprint/65655

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