ISOLASI KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER

MERINT, MERINT and Raudhati, Erfi and Sari, Meisji Liana (2007) ISOLASI KARAKTERISASI BAKTERI ASAM LAKTAT PADA USUS AYAM BROILER. Undergraduate thesis, Sriwijaya University.

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Abstract

The objective of the research was to evaluate and characterisize of a Lactic Acid Bacteria isolated from broiler’s small intestine. The research was done by three steps : (1) Isolated Lactic Acid Bacteria, (2) Gram colourize and microscopic observation and (3) Inhibitory test with pathogen bacteria. It was conducted from March 26 to April 27, 2007 at Microbiology Laboratory of Agriculture Technology Department, Faculty of Agriculture, Sriwijaya University, Indralaya. The isolation of lactic acid bacteria was proceed by clone ffom broiler’s small intestine with MRS Broth for 24 hours, thinned until 10‘9 with serial number, invested in MRS jeli, purify by streak plate method and storaged the lactic acid bacteria in an MRS angle jeli. Gram colourize was done with crystal violet liquid, Iodium, Alcohol 95% and Safranin, and also miroscopic observation was done by electrone microscope with 40 zooming. Inhibitory test with pathogen bacteria was evaluated with Escherichia coli as testing bacteria in 2, 4, 6, 8, 10, 12, 24 and 48 hours incubation period. The isolation result showed two sample of isolate (Al and A2) and consist of the observation in shape as well as colour of the bacteria growth, for sample of isolate Al the colonies had perfect circle shape and white colour. The isolate of A2 had circle shape with mosaic and white broken colour. After that, gramcolouring was done and microscopic observation were identified into gram- negatife bacteria became bacill and bacill cocco. Inhibitory test with pathogen bacteria showed that it produced antimicrobial with 11 mm clearing zone diameter. The result of the research got Lactbacillus sp as the genera of Laclid Acid Bacteria.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	ayam broiler
Subjects:	S Agriculture > SF Animal culture > SF94.5-99 Feeds and feeding. Animal nutrition
Divisions:	05-Faculty of Agriculture > 54231-Animal Husbandry (Animal Science) (S1)
Depositing User:	Mrs Beta Ria Febrianti
Date Deposited:	23 Jun 2022 07:25
Last Modified:	23 Jun 2022 07:25
URI:	http://repository.unsri.ac.id/id/eprint/72794

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