IDENTIFIKASI BAKTERI YANG DIISOLASI DARI Lemna aequinoctialis BERDASARKAN GEN 16S rRNA DAN SUMBANGANNYA PADA PEMBELAJARAN BIOLOGI SMA

NISA, KHOIRUN and Nazip, Khoiron (2023) IDENTIFIKASI BAKTERI YANG DIISOLASI DARI Lemna aequinoctialis BERDASARKAN GEN 16S rRNA DAN SUMBANGANNYA PADA PEMBELAJARAN BIOLOGI SMA. Undergraduate thesis, Sriwijaya Univeristy.

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Abstract

Lemna sp. is a small aquatic plant that is currently receiving a lot of attention because of its potential as an ingredient in animal feed, biological fertilizer, and sustainable food. This research aims to construct the diversity of associated bacteria isolated from Lemna aequinoctialis. Based on identification by using 16S rRNA gene sequences with PCR (Polymerase Chain Reaction) amplification from eight bacteria isolates, they showed high similarity (>98%) comparing to type strain in EzBioCould. They are categorized into three phylum: Proteobacteria (4 isolates), Actinobacteria (3 isolates), and Bacteroidetes (1 isolate). Phylogenetic tree analysis stated that the Bacteriodestes phylum is in the Outgroup branch, or the type of phylum that is most distantly related. The result can be contributed to Biology learning with basic competence 3.5 Identify the structure, habbits, reproduction, and role of bacteria in life presented as a booklet with the results of calculating by Content Validity Ratio (CVR) is 11 and by Content Validity Index (CVI) is 1 in the valid category. Keywords : 16S rRNA Gene, Diversity of Bacteria, Lemna aequinoctialis, Phylogenetic Tree.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Sekuensing, Duckweed, Gen 16S rRNA
Subjects:	L Education > LT Textbooks Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions:	06-Faculty of Education and Educational Science > 84205-Biology Education (S1)
Depositing User:	Khoirun Nisa
Date Deposited:	26 Jul 2023 08:24
Last Modified:	26 Jul 2023 08:24
URI:	http://repository.unsri.ac.id/id/eprint/122333

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