INDUKSI KALUS EKSPLAN DAUN DUKU (Lansium domesticum Corr.) SECARA IN VITRO DENGAN PENAMBAHAN AUKSIN

FAHRUDDIN, FARHAN and Susilawati, Susilawati and Kurnianingsih, Astuti (2015) INDUKSI KALUS EKSPLAN DAUN DUKU (Lansium domesticum Corr.) SECARA IN VITRO DENGAN PENAMBAHAN AUKSIN. Undergraduate thesis, Sriwijaya University.

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Abstract

The aim of this research was to obtain the best callus formation in vitro culture by addition of 2,4-D (2,4-dicholorophenoxyacetic acid) and picloram (4-amino-3,5,6-tricloropicolinic acid) to induced duku’s leaf explants (Lansium domesticum Corr.). This research was conducted from February 2014 to October 2014 at Tissue Cultrue Laboratory, Department of Agronomy, Faculty of Agriculture, Sriwijaya University, Ogan Ilir, Palembang, South Sumatera. The culture medium which was used WPM (woody plant medium) with 2.4-D and picloram treatments and each of them contained of 0 ppm (control), 2 ppm, 4 ppm, and 8 ppm. This research was arranged in completly randomized design with 7 treatments and 5 replications. Each unit of treatment included of 6 culture bottles (sub replication) so that the total were 210 culture bottles. The result showed that duku’s leaf explant could make callus by additon of 2,4-D and picloram. Additin of 2,4-D with 4 ppm concentration had show the best result. The colour of growth callus was white, yellow to brown and also had the crumb texture.

Item Type: Thesis (Undergraduate)
Uncontrolled Keywords: Duku’s leaf explants, Auxin, Callus.
Subjects: T Technology > TJ Mechanical engineering and machinery > TJ1480-1496 Agricultural machinery. Farm machinery
Divisions: 05-Faculty of Agriculture > 54211-Agricultural Technology (S1)
Depositing User: Mrs Sri Astuti
Date Deposited: 14 Nov 2019 03:08
Last Modified: 14 Nov 2019 03:08
URI: http://repository.unsri.ac.id/id/eprint/16201

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