Heat Resistance of Probiotic Candidate Enterococcus faecalis R22B in Different Matrices

Lestari, Shanti Dwita and Rinto, Rinto and Wahyuni, Indah Sri and Ridhowati, Sherly and Wulandari, Wulandari (2021) Heat Resistance of Probiotic Candidate Enterococcus faecalis R22B in Different Matrices. Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, 16 (1). pp. 19-27. ISSN 2089-5690

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Abstract

Fermented fish, including rusip and bekasam, contains lactic acid bacteria, some of which have probiotic properties. Considering the beneficial effect, the count of viable cells in probiotic carrier foods must be at least 106 CFU/g throughout the product shelf-life. The processing and storage condition significantly affect the viability of probiotics in carrier foods. This research was conducted to determine the heat resistance of probiotic candidate Enterococcus faecalis R22B isolated from rusip in three different media: bekasam, rusip, and physiological saline solution (NaCl 0.85%). E. faecalis R22B was heated in each medium at a temperature of 70 °C for 0, 2, 4, 8, and 12 min. The media characteristics showed differences within the parameters included pH values of 4.41-6.99; a w 0.67-0.92; moisture content 72.56-98.09%; ash content 0.69- 12.32%; protein content 0-14.7%; fat content 0-0.84%; and carbohydrate content from 0-4.93%. The results showed that the difference in the heating medium had no significant effect (p>0.05) on the heat resistance of the bacteria, as indicated by the D70 value. The required average heating time at 70 oC to decrease E. faecalis R22B cells by 1log10 (D70 value) in bekasam, rusip, and physiological saline media were 3.17, 3.29, and 3.55 min, respectively. Despite having a lower D T-value, the reduction of bacteria cells in three media heated at 70 oC showed no difference (p>0.05); therefore, bekasam and rusip are suitable for probiotic carrier food.

Item Type:	Article
Uncontrolled Keywords:	bekasam, D value, heat treatment, pasteurization, rusip
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Articles Access for TPAK (Not Open Sources) #3 Repository of Lecturer Academic Credit Systems (TPAK) > Corresponding Author
Divisions:	05-Faculty of Agriculture > 54244-Fisheries Product Technology (S1)
Depositing User:	Shanti Dwita Lestari
Date Deposited:	21 Jul 2025 04:48
Last Modified:	21 Jul 2025 04:48
URI:	http://repository.unsri.ac.id/id/eprint/177586

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