Similarity : NFATc1 releases BCL6-dependent repression of CCR2 agonist expression in peritoneal macrophages from Saccharomyces cerevisiae infected mice

Murti, Krisna and Busch, Rhona and Liu, Jiming and K.Patra, Amiya and Muhammad, Khalid and Knobeloch, Klaus Peter and Lichtinger, Monika and Bonifer, Contanze and Wortge, Simoge and Waisman, Ari and Reifenberg, Kurt and Ellenrieder, Volker and Serfling, Edgar and Avots, Andris (2023) Similarity : NFATc1 releases BCL6-dependent repression of CCR2 agonist expression in peritoneal macrophages from Saccharomyces cerevisiae infected mice. Turnitin Universitas Sriwijaya. (Submitted)

Text NFATc1 releases BCL6-dependent repression of CCR2 agonist expression in peritoneal macrophages from Saccharomyces cerevisiae infected mice.pdf Download (4MB)

Abstract

The link between the extensive usage of calcineurin (CN) inhibitors cyclosporin A and tacrolimus (FK506) in transplantation medicine and the increasing rate of opportunistic infections within this segment of patients is alarming. Currently, how peritoneal infections are favored by these drugs, which impair the activity of several signaling pathways including the Ca++/CN/NFAT, Ca++/CN/cofilin, Ca++/CN/BAD, and NF-κB networks, is unknown. Here, we show that Saccharomyces cerevisiae infection of peritoneal resident macrophages triggers the transient nuclear translocation of NFATc1β isoforms, resulting in a coordinated, CN-dependent induction of the Ccl2, Ccl7, and Ccl12 genes, all encoding CCR2 agonists. CN inhibitors block the CCR2-dependent recruitment of inflammatory monocytes (IM) to the peritoneal cavities of S. cerevisiae infected mice. In myeloid cells, NFATc1/β proteins represent the most prominent NFATc1 isoforms. NFATc1/β ablation leads to a decrease of CCR2 chemokines, impaired mobilization of IMs, and delayed clearance of infection. We show that, upon binding to a composite NFAT/BCL6 regulatory element within the Ccl2 promoter, NFATc1/β proteins release the BCL6-dependent repression of Ccl2 gene in macrophages. These findings suggest a novel CN-dependent cross-talk between NFAT and BCL6 transcription factors, which may affect the outcome of opportunistic fungal infections in immunocompromised patients.

Item Type:	Other
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Results of Ithenticate Plagiarism and Similarity Checker
Divisions:	04-Faculty of Medicine > 11718-Pathology Anatomy (Sp
Depositing User:	dr., Ph.D. Krisna Murti
Date Deposited:	11 May 2023 07:43
Last Modified:	11 May 2023 07:43
URI:	http://repository.unsri.ac.id/id/eprint/101448

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