Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method

Muhammad Yerizam, yerizam and ASYENI MIFTAHUL JANNAH, ASYENI and Nabila Aprianti, Nabila (2023) Bioethanol Production from Chlorella Pyrenoidosa by Using Enzymatic Hydrolysis and Fermentation Method. Journal of Ecological Engineering, 24 (1). pp. 34-40. ISSN 2299–8993

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Abstract

Starch can be found in microalgae, the raw material for the third generation of bioethanol production. One of them is C. pyrenoidosa. This study was conducted to analyze the effect of α-amylase enzyme concentration on the glucose contents produced and the effects of fermentation time on bioethanol contents produced. The hydrolysis process of this study was conducted using an α-amylase enzyme produced by A. niger. Several analyses in this research were carried out, including the analysis of enzyme activity using the Fuwa method, the analysis of glu�cose levels from enzymatic hydrolysis using the DNS method, and the analysis of bioethanol contents using the density method and GC-MS. The highest glucose content was 0.67 mg/mL, which was obtained from the addition of 40% (v/w) α-amylase enzyme, and the yield of bioethanol content from the sample treated 40% (v/w) α-amylase enzyme and fermented for 9 days was the optimum, which produced 28.07% of bioethanol content.

Item Type:	Article
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Articles Access for TPAK (Not Open Sources)
Divisions:	03-Faculty of Engineering > 24201-Chemical Engineering (S1)
Depositing User:	Asyeni Miftahul Jannah
Date Deposited:	12 Jul 2023 03:03
Last Modified:	12 Jul 2023 03:03
URI:	http://repository.unsri.ac.id/id/eprint/115928

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