Safe 2023, Bool of Programs, International Conference on Sustainable Agriculture, Food, and Energy, SCREENING OF LOCAL VARIETIES OF RICE (Oryza Sativa L.). SOUTH SUMATRA USING PEG 6000 SOLUTION AND THE MOLECULAR MARKER METHOD (SSR)

Hasmeda, Mery and Adriansyah, Fikri and ALHAMAMI, ZENDI (2023) Safe 2023, Bool of Programs, International Conference on Sustainable Agriculture, Food, and Energy, SCREENING OF LOCAL VARIETIES OF RICE (Oryza Sativa L.). SOUTH SUMATRA USING PEG 6000 SOLUTION AND THE MOLECULAR MARKER METHOD (SSR). In: SAFE 2023, 28 May 2023, Chiang Mai.

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Abstract

The aims of this study was to find local rice South Sumatra varieties that was resistant to drought stress using Polyethylene Glycol (PEG) and PCR-RAPD solutions. This research was conducted at the Agricultural Cultivation laboratory, Faculty of Agriculture, Sriwijaya University, North Indralaya District, Ogan Ilir Regency, South Sumatra. This study used 2 factors, the first factor is Aquades solution as control, PEG with a concentration of 10%, and PEG with a concentration of 20%. The second factor, namely PCR-RAPD, was used by combining PCR techniques with random sequens primers for the purpose of genome random locus amplication. The parameters being observed were sprout length, bud length, main root length, seminal root length, germination percentage, dry weight of shoots, dry weight of roots, vigor index and PCR-RAPD amplication results. The results of PEG research showed that solar rice, golden solar, tehas and short varieties were tolerant of drought stress. DNA amplification showed that DNA bands on each marker against solar rice, golden solar, tehas and short varieties were identical to comparison variety genes that are resistant to drought stress.

Item Type:	Conference or Workshop Item (Paper)
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Conference or Workshop
Divisions:	05-Faculty of Agriculture > 54204-Agronomy (Agricultural Sciences) (S1)
Depositing User:	Fikri Adriansyah
Date Deposited:	18 Jul 2023 13:30
Last Modified:	18 Jul 2023 13:30
URI:	http://repository.unsri.ac.id/id/eprint/117459

Available Versions of this Item

Safe 2023, Bool of Programs, International Conference on Sustainable Agriculture, Food, and Energy, SCREENING OF LOCAL VARIETIES OF RICE (Oryza Sativa L.). SOUTH SUMATRA USING PEG 6000 SOLUTION AND THE MOLECULAR MARKER METHOD (SSR). (deposited 18 Jul 2023 13:30) [Currently Displayed]
- Safe 2023, Book of Programs, International Conference on Sustainable Agriculture, Food, and Energy, SCREENING OF LOCAL VARIETIES OF RICE (Oryza Sativa L.). SOUTH SUMATRA USING PEG 6000 SOLUTION AND THE MOLECULAR MARKER METHOD (SSR). (deposited 18 Jul 2023 13:31)

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