UJI KADAR TOTAL FENOL, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETANOL BATANG KAYU KUNING (ARCANGELISIA FLAVA)

MULAWARMAN, HISHAM and Fatmawati, Fatmawati and Oktharina, Eka Handayani (2024) UJI KADAR TOTAL FENOL, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN FRAKSI ETANOL BATANG KAYU KUNING (ARCANGELISIA FLAVA). Undergraduate thesis, Sriwijaya University.

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Abstract

This study examines the total phenol content, flavonoid levels, and antioxidant capacity of the ethanol fraction of Arcangelisia flava (Yellow Wood). Oxidative stress, caused by an imbalance between reactive oxygen species (ROS) and antioxidants, is a major factor in various chronic diseases. Arcangelisia flava, commonly used in traditional Southeast Asian medicine, contains secondary metabolites such as phenols and flavonoids, which have potential antioxidant properties. Fractionation was carried out using maceration with solvents based on polarity, including n-hexane, ethyl acetate, and ethanol. Total phenol and flavonoid content were measured at concentrations of 20 ppm, 40 ppm, 60 ppm, 80 ppm, and 100 ppm. Phenol content was determined using the Folin-Ciocalteu method, while flavonoid content was assessed using the quercetin method. Antioxidant activity was evaluated using six concentrations—2 ppm, 5 ppm, 10 ppm, 15 ppm, 20 ppm, and 25 ppm—through DPPH radical scavenging, and the IC50 value was calculated to assess the antioxidant strength of the ethanol fraction. The ethanol fraction of Arcangelisia flava stem exhibited significant phenol and flavonoid content, with values of 57.807 ± 3.283 mg GAE/g and 114.642 ± 1.015 mg QuE/g, respectively. Antioxidant activity, as measured by the DPPH assay, indicated a strong antioxidant capacity with an IC50 value of 72.876 ppm. The ethanol fraction of Arcangelisia flava stem contains high levels of phenols and flavonoids and exhibits potent antioxidant activity, suggesting its potential in supporting the use of herbal ingredients for disease treatment and prevention. Keywords: Arcangelisia flava, total phenol content, total flavonoid content, antioxidant

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Arcangelisia flava, kadar total fenol, kadar total flavonoid, antioksidan
Subjects:	R Medicine > R Medicine (General) > R5-920 Medicine (General)
Divisions:	04-Faculty of Medicine > 11201-Medicine (S1)
Depositing User:	Hisham Mulawarman
Date Deposited:	06 Dec 2024 05:37
Last Modified:	06 Dec 2024 05:37
URI:	http://repository.unsri.ac.id/id/eprint/160057

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