ALELOPATI TANAMAN TEMU KUNCI (Boesenbergia pandurata) TERHADAP Ganoderma boninense PATOGEN BUSUK PANGKAL BATANG KELAPA SAWIT

TRIANI, LUSY and Suwandi, Suwandi (2024) ALELOPATI TANAMAN TEMU KUNCI (Boesenbergia pandurata) TERHADAP Ganoderma boninense PATOGEN BUSUK PANGKAL BATANG KELAPA SAWIT. Undergraduate thesis, Sriwijaya University.

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Abstract

BSR (Basal Stem Rot) disease can cause the productive lifespan of oil palm plants to be shorter than the typical productive lifespan of oil palms. Ganoderma boninese is a soil-borne pathogenic fungus that can infect oil palm plants through their roots and spread the disease by producing basidiospores. Based on the background above, the problem formulation of this research is how the rhizome exudate of temu kunci affects the growth of the fungus G. boninense, the color change of tanin, EC, and pH of the MEA (Malt Extract Agar) + tanin medium. The objective of this research is to determine the effect of the rhizome exudate of temu kunci on the growth of the fungus G. boninense, the color change of tanin, EC, and pH of the MEA (Malt Extract Agar) + tanin medium. In this test, one type of medium is used, namely the MEA (Malt Extract Agar) + tanin medium. In each medium, the RAL method was used, consisting of 4 treatments with 5 repetitions and 5 exudate samples. The treatments used in this study were the exudate of temu kunci with concentrations of 0% or control, 1.25%, 5%, and 20%. To obtain the exudate from the temu kunci plant (Boesenbergia pandurata), one-month-old plants were uprooted, the roots were washed with running water, then soaked in 500 ml of distilled water and extracted for 12 hours using an aerator. The obtained exudate was sterilized through a three-stage filtration: aquarium filter, coffee filter paper, and Whatman No. 1 filter paper. The exudate test media was prepared by mixing MEA media and exudate, including tanin media with exudate concentrations of 20%, 5%, 1.25%, and control. Preparation of liquid media by mixing 2 g of malt extract and 2 g of agar per bottle, adding 0.5 g of tanin, then each bottle filled with aquadest according to concentration, covered with aluminum foil, and sterilized in an autoclave at 121°C for 2 hours. After sterilization, the media is poured under sterile conditions in a laminar air flow, and each bottle is given antibiotics, with five repetitions per treatment. The planting of G. boninense fungus was carried out after the isolate was five days old, using a cork borer to ensure uniform size, and then the mushroom pieces were planted in the center of the medium. The media were tightly covered with plastic wrap to prevent contamination, labeled according to treatment, and observed daily for five to seven days. The test results showed that the inhibition from each exudate and concentration had different inhibition values. Each extract of temu kunci (Boesenbergia pandurata) has both negative and positive values on the growth of the pathogen G. boninense. The allelopathy of TK1E is negative because the values of colony growth, tanin oxidation, EC value, and pH are significantly different from the control. The presence of a 20.8% inhibition at a concentration of 5%, increased oxidation at a concentration of 20%, and higher EC at a concentration of 5% while pH remained unaffected. Allelopathy TK1 is positive towards colony growth, tanin oxidation, EC value, and pH, with the medium not significantly different from the control. There is an 18.9% inhibition at a concentration of 1.25%, increased oxidation at a concentration of 5%, and higher EC at a concentration of 1.25% while pH remained unaffected. The allelopathic activity of TK2 depends on the concentration; at a concentration of 1.25%, allelopathy is negative due to a 42.5% inhibition, whereas at a concentration of 20%, allelopathy is positive for colony growth. Tanin oxidation and the EC and pH values of the media are not affected. Allelopathy TKK2 is positive for colony growth because there is only an inhibition value at a concentration of 20 of 3.6%, but negative for the media EC value, while tanin oxidation and media pH value are not affected. Allelopathy TKR1 is positive for colony growth because inhibition only occurs at a concentration of 5% with a value of 13.5%, but negative for the media EC value. Meanwhile, tanin oxidation and media pH value are not affected. The conclusion of this study is that the rhizome exudate of temu kunci (Boesenbergia pandurata) has a positive allelopathic effect on colony growth. The rhizome exudate of temu kunci causes a decrease in EC in the growth medium of G. boninense. The decolorization of tanin color and the pH of the medium are not affected by the rhizome exudate of temu kunci. The recommendation in this study is to conduct further research on the allelopathic test of the rhizome exudate of temu kunci (Boesenbergia pandurata) on the growth of colonies and the decolorization of tanin color in G. boninense. Keywords: Allelopathy; Boesenbergia pandurata; Oil Palm; Ganoderma boninense.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Allelopathy; Boesenbergia pandurata; Oil Palm; Ganoderma boninense
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General)
Divisions:	05-Faculty of Agriculture > 54295-Plant Protection (S1)
Depositing User:	Lusy Triani
Date Deposited:	23 Jan 2025 02:58
Last Modified:	23 Jan 2025 02:58
URI:	http://repository.unsri.ac.id/id/eprint/166529

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