FLAVONOID APIGENIN DARI EKSTRAK DAUN TUMBUHAN RUKAM (Flacourtia rukam) SERTA UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI

MAHANISA, ANI SARAH and Muharni, Muharni and Elfita, Elfita (2020) FLAVONOID APIGENIN DARI EKSTRAK DAUN TUMBUHAN RUKAM (Flacourtia rukam) SERTA UJI AKTIVITAS ANTIOKSIDAN DAN ANTIBAKTERI. Undergraduate thesis, Sriwijaya University.

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Abstract

Rukam Plant (Flacourtia rukam) belongs to Flacourtia which has pharmacological activity as an antioxidant and antibacterial, where one of the benefits of the leaves used to treatment of eye pain, but there is no information on the chemical content of the leaves of the Flacourtia rukam. This study aimed to isolation flavonoid compound and biological activity antioxidant and antibacterial assay. This research was started by maceration using solvents with increasing polarity then separation and purification by chromatographic techniques. The structure of the compound was determined based on spectroscopic data analysis which included UV-Vis, FT-IR, NMR 1D (1H-NMR amd 13C-NMR), and NMR 2D (HMBC and HSQC) spectrometry. Antioxidant activity was tested using the DPPH method (1,1-diphenyl-2-picril hydrazil) and antibacterial using diffusion method of against Escherichia coli, Shigella dysenteriae, Staphlococcus aereus, and Bacillus subtilis. One pure compound was isolated form yellow crystal (18 mg). Based on spectroscopic data analysis and comparing with the literature data, the compound belong to flavonoid group that is apigenin. The pure compound has an IC50 value of 19.13 mg/L and categorized as an active antioxidant activity while the antibacterial pure compound can inhibit the bacteria E.coli, S.dysenteriae, S.aereus, and B. subtilis at concentrations of 1000, 500, 250, 125, 62.5 µg / mL and have an average inhibition zone value 7-10 mm included in the medium category.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Antioksidan, antibakteri, apigenin, Flacourtia rukam
Subjects:	Q Science > QD Chemistry > QD241-441 Organic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Users 4644 not found.
Date Deposited:	27 Jan 2020 07:09
Last Modified:	27 Jan 2020 07:09
URI:	http://repository.unsri.ac.id/id/eprint/25818

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