DNA BARCODE IKAN BELIDA (Chitala sp.) BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI)

ANJARSARI, YULIANTI and Syaifudin, Mochamad and Jubaedah, Dade (2020) DNA BARCODE IKAN BELIDA (Chitala sp.) BERDASARKAN GEN SITOKROM C OKSIDASE SUBUNIT I (COI). Undergraduate thesis, Sriwijaya University.

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Abstract

Featherbacks (Chitala lopis) in Indonesia inhabit riverine of Sumatra, Java and Kalimantan. Featherbacks have been protected as threatened species. This research aims to identify the sequence of Cytochrome C Oxidase Subunit I (COI) gene of mtDNA, construct the phylogenetic trees among species of featherbacks and know the physical chemical of water characteristics of featherbacks habitat in the Musi River at Beruge village, Babat Toman sub-district, Musi Banyuasin regency and featherbacks at PT PLN Persero Indralaya, Ogan Ilir regency. This research was conducted in March–August 2020. The method used in barcoding species and determining phylogenetic were DNA isolation, DNA amplification using PCR and sequencing of COI gene regions of mtDNA. Squencing of COI gene regions of mtDNA from the fish samples were collected from Musi River and PT PLN. The COI gene was sequenced by PCR (Polymerase Chain Reaction) with optimum annealing temperature 50oC for 30 second with 35 cycles. After editting, sequence length of the COI gene of Chitala sp. was 621 base pairs (bp) nucleotide. Based on BLAST analysis compared to Genbank database, Chitala sp. from PT PLN Persero Indralaya CLP 2 had the highest similarity of 99.28% with C. lopis (Malaysia) and 98.88% from Kampar river, Riau. Chitala sp. from Musi River (CLS 1, CLS 3) and PT PLN (CLP 3) had the highest similarity of 95.19% with C. chitala from India. Physical chemical of water characteristics of Chitala sp. in Musi River and PT PLN Persero Indralaya were temperature 28.7-30.2 oC, transparency 12-26 cm, dissolved oxygen 6.31-7.66 mg L-1 , pH 6-7.80, ammonia 0.03-1.31 mg L-1 and total alkalinity 16-60 mg L-1 CaCO3. Keywords: Cytochrome C Oxidase Subunit I gene, Chitala sp., phylogenetic.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Melihat kekerabatan ikan belida di Sumatera Selatan
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH1-691 Aquaculture. Fisheries. Angling S Agriculture > SH Aquaculture. Fisheries. Angling > SH327.5-327.7 Fishery resources. Fishery conservation
Divisions:	05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User:	Users 8849 not found.
Date Deposited:	12 Nov 2020 07:08
Last Modified:	12 Nov 2020 07:08
URI:	http://repository.unsri.ac.id/id/eprint/37515

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