Hermansyah, Hermansyah and Susilawati, Susilawati (2017) GENE EXPRESSION CHANGES AND ANTI-PROLIFERATIVE EFFECT OF NONI (Morinda citrifolia) FRUIT EXTRACT ANALYSED BY REAL TIME-PCR. MOLEKUL, 12 (1). pp. 37-44. ISSN 2503-0310
Preview |
Text
GENE EXPRESSION CHANGES AND ANTI-PROLIFERATIVE EFFECT OF NONI (Morinda citrifolia) FRUIT EXTRACT ANALYSED BY REAL TIME-PCR .pdf Download (578kB) | Preview |
Abstract
To elucidate the anti-proliferative effect of noni (Morinda citrifolia) fruit extract for a Saccharomyces cerevisiae model organism, analysis of gene expression changes related to cell cycle associated with inhibition effect of noni fruit extract was carried out. Anti-proliferative of noni fruit extract was analyzed using gene expression changes of Saccharomyces cerevisiae (strains FY833 and BY4741). Transcriptional analysis of genes that play a role in cell cycle was conducted by growing cells on YPDAde broth medium containing 1% (w/v) noni fruit extract, and then subjected using quantitative real-time polymerase chain reaction (RT-PCR). Transcriptional level of genes CDC6 (Cell Division Cycle-6), CDC20 (Cell Division Cycle-20), FAR1 (Factor ARrest-1), FUS3 (FUSsion-3), SIC1 (Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase-1), WHI5 (WHIskey-5), YOX1 (Yeast homeobOX-1) and YHP1 (Yeast Homeo-Protein-1) increased, oppositely genes expression of DBF4 (DumbBell Forming), MCM1 (Mini Chromosome Maintenance-1) and TAH11 (Topo-A Hypersensitive- 11) decreased, while the expression level of genes CDC7 (Cell Division Cycle-7), MBP1 (MIul-box Binding Protein-1) and SWI6 (SWItching deficient-6) relatively unchanged. These results indicated that gene expression changes might associate with anti-proliferative effect from noni fruit extract. These gene expressions changes lead to the growth inhibition of S.cerevisiae cell because of cell cycle defect.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD415-436 Biochemistry |
| Depositing User: | Hermansyah Hermansyah |
| Date Deposited: | 20 Sep 2021 05:47 |
| Last Modified: | 20 Sep 2021 05:47 |
| URI: | http://repository.unsri.ac.id/id/eprint/54312 |
Actions (login required)
 |
View Item |