UJI AKTIVITAS ENZIM AZOREDUKTASE EKSTRASELULER DARI BAKTERI PSEUDOMONAS STUTZERI

ZUHRO, SITI and Julinar, Julinar and Hidayati, Nurlisa (2022) UJI AKTIVITAS ENZIM AZOREDUKTASE EKSTRASELULER DARI BAKTERI PSEUDOMONAS STUTZERI. Undergraduate thesis, Sriwijaya University.

	Text RAMA_47201_08031281722028.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_47201_08031281722028_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (3MB) | Request a copy
Preview	Text RAMA_47201_08031281722028_0025076501_0009117201_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (1MB) | Preview
	Text RAMA_47201_08031282722028_0025076501_0009117201_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (499kB) | Request a copy
	Text RAMA_47201_08031281722028_0025076501_0009117201_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (435kB) | Request a copy
	Text RAMA_47201_08031281722028_0025076501_0009117201_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (184kB) | Request a copy
	Text RAMA_47201_08031281722028_0025076501_0009117201_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (415kB) | Request a copy
	Text RAMA_47201_08031281722028_0025076501_0009117201_06_ref.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (322kB) | Request a copy
	Text RAMA_47201_08031281722028_0025076501_0009117201_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (736kB) | Request a copy

Abstract

Extracellular azoreductase enzyme activity test has been carried out from the Pseudomonas stutzeri. P. stutzeri is bacteria bacteria that have the ability to decolorize azo dyes against methyl red dyes.This study aimed to examine the activity of the extracellular azoreductase enzyme from P. stutzeri. The azoreductase enzyme extract from P. Stutzeri was obtained by centrifugation of the liquid culture on NB media which was incubated for 2x24 hours at 37◦C in an incubator shaker. The results of the selected fractions were used to calculate enzyme activity and protein content. Determination of the optimum conditions for the extracellular azoreductase enzyme was carried out at variations in incubation time, pH, temperature, substrate concentration and calculation of enzyme kinetics Vmax and Km. The highest fraction was fraction 3 with a saturation level of 40%-60% with an enzyme activity of 0.1101 Unit/mL and a specific enzyme activity of 0.1079 U/mg. Extracellular Azoreductase enzyme from P. stutzeri has optimum conditions at 5 minutes incubation time, pH 7, temperature 37ºC and substrate concentration 70µM with enzyme activity 0.1166 U/mL. The values of Vmax and Km obtained are 0.173683 U/mL and 31,33415 M, respectively.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Azoreduktase, Pseudomonas stutzeri, Metil merah
Subjects:	Q Science > QD Chemistry > QD415-436 Biochemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Miss Siti Zuhro
Date Deposited:	20 Jan 2022 05:11
Last Modified:	20 Jan 2022 05:11
URI:	http://repository.unsri.ac.id/id/eprint/61966

Actions (login required)

View Item