HIDROLISIS ENZIMATIK JERAMI PADI DENGAN ENZIM SELULASE DARI Aspergillus niger DAN Trichoderma reesei PADA PEMBUATAN BIOETANOL

TRIANI, IIS and Hermansyah, Hermansyah and Julinar, Julinar (2014) HIDROLISIS ENZIMATIK JERAMI PADI DENGAN ENZIM SELULASE DARI Aspergillus niger DAN Trichoderma reesei PADA PEMBUATAN BIOETANOL. Undergraduate thesis, Sriwijaya University.

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Abstract

The research about bioethanol production as altemative energy resource has been done from rice straw. Cellulose content in rice straw can be used as substrate to produce cellulase enzymes by Aspergillus niger and Trichoderma reesei each other with solid State fermentation at pH 5 with 30° C temperature. This research was done in four steps. Delignification with 2 % NaOH to reduce lignin content, production of cellulase enzymes, hydrolysis with cellulase enzymes to produce glucose, and fermentation with Saccharomyces cerevisiae to produce ethanol. The result of research showed that delignification with 2 % NaOH reduced lignin as much as 36.59 %. The highest protein content and the highest enzyme activity were produced by cellulase enzyme extract from Trichoderma reesei as much as 9.728 mg/mL and 2.392 IU/mL was obtained at 7 days fermentation time, the highest glucose content was produced from hydrolysis by cellulase enzyme extract from Trichoderma reesei as much as 16.390 mg/mL was obtained at 5 days incubation time, and the highest ethanol volume was produced by fermentation from the highest hydrolysis with cellulase enzyme extract from Aspergillus niger was 9.379 mL respectively measured by gas chromatography.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Rice straw, Aspergillus niger, Trichoderma reesei, cellulase, Saccharomyces cerevisiae, ethanol.
Subjects:	Q Science > QD Chemistry > QD146-197 Inorganic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	04 Mar 2022 01:50
Last Modified:	04 Mar 2022 01:50
URI:	http://repository.unsri.ac.id/id/eprint/65741

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