ISOLASI SENYAWA METABOLIT SEKUNDER DARI JAMUR ENDOFITIK Fusarium sp RIMPANG TUMBUHAN KUNYIT PUTIH (Curcuma zedoaria (Berg.) Roscoe) DAN UJI AKTIVITAS ANTIBAKTERINYA

RULIZA, MILANTI OKTA and Muharni, Muharni and Elfita, Elfita (2013) ISOLASI SENYAWA METABOLIT SEKUNDER DARI JAMUR ENDOFITIK Fusarium sp RIMPANG TUMBUHAN KUNYIT PUTIH (Curcuma zedoaria (Berg.) Roscoe) DAN UJI AKTIVITAS ANTIBAKTERINYA. Undergraduate thesis, Sriwijaya University.

	Text RAMA_47201_08091003008.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (45MB) | Request a copy
Preview	Text RAMA_47201_08091003008_0004036903_0026036901_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (15MB) | Preview
	Text RAMA_47201_08091003008_0004036903_0026036901_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (6MB) | Request a copy
	Text RAMA_47201_08091003008_0004036903_0026036901_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (3MB) | Request a copy
	Text RAMA_47201_08091003008_0004036903_0026036901_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (14MB) | Request a copy
	Text RAMA_47201_08091003008_0004036903_0026036901_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (496kB) | Request a copy
	Text RAMA_47201_08091003008_0004036903_0026036901_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (2MB) | Request a copy
	Text RAMA_47201_08091003008_0004036903_0026036901_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (5MB) | Request a copy

Abstract

The secondary metabolite compound from endophytic fungi Fusarium sp of kunyit putih (Curcuma zedoaria (Berg) Roscoe) rhizome plant have been isolated. Isolation began with cultivation of Fusarium sp fungi in 8 L of PDB’s medium (Potato Dextrose Broth) until 21 days. The medium culture was extracted partition used ethyl acetate and then was evaporated. The ethyl acetate extract was separated and purified by chromatography techniques. The pure compound was obtained and then antibacterial activity was tested. Antibacterial activity by disc diffusion method was tested using bacterial test E. coli, S. dysentriae, S. aureus, and B.subtilis. This compound in the form of yellow solid (0.36 g) with melting point 171-172°C. Base on spectroscopy analysis data UV, IR, and NMR showed that result of isolation compound was 5 (4’-ethoxy-2,-hydroxy-5,-methyl-2’,3’- dihydrofuran-3’-yl (hydroxy) methyl-4-isoprophyl-3-methyl-2-pyran-2-on). The structure of isolated compound still has some infirmity and needs to be measured at NOESY and MS. The isolated compound showed antibacterial activity equivalent to the Ampicilin positive control against bacterial test E. coli and S. aureus at a concentration of 500 ppm with inhibition zone diameter respectively 11 mm. Based on these data concluded isolated compound potentially active antibacterial compound.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Curcuma zedoaria (Berg) Roscoe, Endophytic fungi, Antibacterial
Subjects:	Q Science > QD Chemistry > QD241-441 Organic chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	18 Mar 2022 06:32
Last Modified:	18 Mar 2022 06:32
URI:	http://repository.unsri.ac.id/id/eprint/66279

Actions (login required)

View Item