FORMULASI DAN KARAKTERISASI SOLID LIPID NANOPARTICLES ASAM STEARAT DAN KOSURFAKTAN PEG 400 DENGAN VARIASI JUMLAH GLIBENKLAMID

APRIYANTO, ANDRE AGUNG and Sinulingga, Sadakata and Mardiyanto, Mardiyanto (2022) FORMULASI DAN KARAKTERISASI SOLID LIPID NANOPARTICLES ASAM STEARAT DAN KOSURFAKTAN PEG 400 DENGAN VARIASI JUMLAH GLIBENKLAMID. Undergraduate thesis, Sriwijaya University.

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Abstract

Drug solubility is one of important parameter that will affect on drug absorption and drug bioavailability in body. Glibenclamide is belongs to BCS class II which has good permeability in human body but has a low solubility in water. Low solubility of glibenclamide means that only small amount of glibenclamide can be absorbed through the gastrointestinal. One of solutions to increase solubility of glibenclamid is to be formulated into solid lipid nanoparticles (SLN). Preparation of SLN glibenclamide used high-speed homogenization and ultasonication method. The SLN glibenclamide formula consisted of stearic acid, tween 80, PEG 400, PVA, and glibenclamide were varied amounts to 0,1 g (F1), 0,2 g (F2), and 0,3 g (F3). The best formula was determinated from the EE percentage (%EE). The results %EE against 3 formula were 97,5064 ± 0,0286%, 97,4331 ± 0,0256%, and 97,4049 ± 0,0100%. The results of the heating-cooling cycle stability test for the three formulas showed that there was an increase in pH in each cycle, the result was a slightly cloudy white, there was precipitates, and in F2 and F3 there was a phase seperation. Mechanical stability testing is obtained in the three formulas there is a precipitate. The best formula was obtained in F1 with the amount of glibenclamide 0,1 g which was then carried out with particle characterization and solubility test. The particle characterization results showed average particle diameter 553,6 ± 131 nm, PDI 0,460 ± 0,017, and zeta potential -12,14 ± 0,2 mV. The results of the solubility test showed that SLN glibenclamide was soluble in aquadest, HCl 5%, SGF and SIF. While the NaOH 5% and NaHCO3 5% have slightlty insoluble in the medium. The results showed that glibenclamide could be formulated into soluble SLN. Keywords: Glibenclamide, SLN, Stearic Acid, PEG 400, Percent Encapsulation Efficiency Kelarutan bahan obat menjadi salah satu parameter penting yang akan mempengaruhi absorbsi dan bioavailabilitas obat di dalam tubuh. Glibenklamid termasuk ke dalam BCS kelas II yang memiliki kelarutan rendah di dalam air. Kelarutan glibenklamid yang rendah menyebabkan hanya sedikit glibenklamid yang bisa diserap melalui gastrointestinal. Salah satu solusi untuk meningkatkan kelarutan glibenklamid dengan diformulasi menjadi suatu solid lipid nanoparticles (SLN). Pembuatan SLN glibenklamid dilakukan dengan metode pengadukan kecepatan tinggi dan ultrasonikasi. Formula SLN glibenklamid terdiri dari asam stearat, tween 80, PEG 400, PVA, dan glibenklamid yang divariasikan jumlahnya menjadi 0,1 g (F1), 0,2 g (F2), dan 0,3 g (F3). Formula terbaik akan ditentukan dari nilai persen EE (%EE). Hasil pengujian %EE terhadap 3 formula berturut turut sebesar 97,5064 ± 0,0286%, 97,4331 ± 0,0256%, dan 97,4049 ± 0,0100%. Hasil pengujian stabilitas heating-cooling cycle terhadap ketiga formula didapat terjadi kenaikan pH pada tiap siklus, hasil sediaan berwarna putih sedikit keruh, terdapat endapan, serta pada F2 dan F3 terjadi pemisahan fase. Pengujian stabilitas mekanik didapat pada ketiga formula terdapat endapan. Formula terbaik yang didapat pada F1 dengan jumlah glibenklamid 0,1 g yang selanjutnya dilakukan karakterisasi partikel dan uji kelarutan. Hasil karakterisasi partikel menunjukkan rata-rata diameter partikel sebesar 553,6 ± 131 nm, PDI 0,460 ± 0,017, dan zeta potensial - 12,14 ± 0,2 mV. Hasil uji kelarutan menunjukkan SLN glibenklamid larut dalam pelarut aquadest, HCl 5%, SGF, dan SIF. Sedangkan pada pelarut NaOH 5% dan NaHCO3 5% memiliki kelarutan yang sedikit tidak larut dalam medium. Hasil penelitian menunjukkan bahwa glibenklamid dapat diformulasi menjadi SLN yang larut. Kata kunci: Glibenklamid, SLN, Asam Stearat, PEG 400, Persen Efisiensi Enkapsulas

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Teknologi Farmasi
Subjects:	R Medicine > RS Pharmacy and materia medica > RS192-199 Pharmaceutical technology
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Users 21106 not found.
Date Deposited:	02 Jun 2022 03:04
Last Modified:	02 Jun 2022 03:04
URI:	http://repository.unsri.ac.id/id/eprint/71191

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