UJI AKTIVITAS FRAKSI DAN SENYAWA ANTIBAKTERI DAUN PEPAYA (Carica papaya L.) DAN PENENTUAN KONSENTRASI HAMBAT MINIMUM (KHM) TERHADAP BAKTERI Escherichia coli DAN Salmonella typhi SECARA IN VITRO

NABABAN, ALVIONITA and Salni, Salni and Aminasih, Nita (2014) UJI AKTIVITAS FRAKSI DAN SENYAWA ANTIBAKTERI DAUN PEPAYA (Carica papaya L.) DAN PENENTUAN KONSENTRASI HAMBAT MINIMUM (KHM) TERHADAP BAKTERI Escherichia coli DAN Salmonella typhi SECARA IN VITRO. Undergraduate thesis, Sriwijaya University.

	Text RAMA_46201_08101004012.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (43MB) | Request a copy
Preview	Text RAMA_46201_08101004012_0023086604_0007056211_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (13MB) | Preview
	Text RAMA_46201_08101004012_0023086604_0007056211_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (9MB) | Request a copy
	Text RAMA_46201_08101004012_0023086604_0007056211_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (5MB) | Request a copy
	Text RAMA_46201_08101004012_0023086604_0007056211_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (9MB) | Request a copy
	Text RAMA_46201_08101004012_0023086604_0007056211_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (496kB) | Request a copy
	Text RAMA_46201_08101004012_0023086604_0007056211_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (3MB) | Request a copy
	Text RAMA_46201_08101004012_0023086604_0007056211_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (5MB) | Request a copy

Abstract

Papaya (Carica papaya L.) can be used as an altemative to treat diarrheal disease caused by infection with bacteria, such as Escherichia coli and Salmonella typhi. This study aims to know the potential of fractions and compounds of papaya leaves (Carica papaya L.) as an antibacterial to Escherichia coli and Salmonella typhi. The study was conducted in the Laboratory of Genetics and Biotechnology Department of Biology, Faculty of Mathematics and Natural Sciences, University of Sriwijaya, Indralaya, from February to October 2014. The experimental research method using a completely randomized design with 6 treatments and 4 replications, the treatment used were the most active fraction with various concemtration of the active fraction with various concentrations (4000, 2000, 1000, 500, 250, and 125) ug/ml. Extraction was done by maceration method, fractionation was conducted using liquid-liquid ffactionation (FCC), and activity assay test using the agar diffusion method. The test used were Escherichia coli and Salmonella typhi. Data were analyzed with Standard deviation (Sd) and Analysis of Variants (ANOVA) and advanced test using SPSS version 16. The results obtained methanol-water active fraction to bacterial water testing whereas the fraction of ethyl acetate and n-hexane fraction were inactivity. MIC values on methanol-water fraction to Escherichia coli and Salmonella typhi was 250 pg/ml. Water methanol fraction containing antibacterial flavonoid compounds. MIC values of compounds active to Escherichia coli and Salmonella typhi was 125 pg / ml. From this study it can be concluded that the methanol-water fraction of water from the leaves of papaya (Carica papaya L.) has antibacterial activity to Escherichia coli and Salmonella typhi.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Carica papaya L., antibacterial, minimum inhibilory concentraiion
Subjects:	Q Science > QR Microbiology > QR75-99.5 Bacteria
Divisions:	08-Faculty of Mathematics and Natural Science > 46201-Biology (S1)
Depositing User:	Mrs Furika Furika
Date Deposited:	16 Jun 2022 08:38
Last Modified:	16 Jun 2022 08:38
URI:	http://repository.unsri.ac.id/id/eprint/72384

Actions (login required)

View Item