PENGARUH MINYAK SACHA INCHI (Plukenetia volubilis L.) TERHADAP PERTUMBUHAN Lactobacillus bulgaricus DAN UJI DAYA HAMBAT TERHADAP Escherichia coli

SEMBIRING, NINA CAMELIA and Miksusanti, Miksusanti and Apriani, Elsa Fitria (2022) PENGARUH MINYAK SACHA INCHI (Plukenetia volubilis L.) TERHADAP PERTUMBUHAN Lactobacillus bulgaricus DAN UJI DAYA HAMBAT TERHADAP Escherichia coli. Undergraduate thesis, Sriwijaya University.

	Text RAMA_48201_08061281823055.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (2MB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_01_front_ref.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (607kB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (612kB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (443kB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (205kB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (477kB) | Request a copy
	Text RAMA_48201_08061281823055_0023076802_0014049204_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy
	Text RAMA_48201_08061281823055_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (5MB) | Request a copy

Abstract

Sacha Inchi Oil (SIO) is a functional food that contains fatty acids that are good for consumption as an alternative in improving health. This study was conducted to see effect giving SIO on the growth of L. bulgaricus in the body and to observe effect of giving L. bulgaricus in combination with SIO as an antibacterial. The growth test of L. bulgaricus was carried out by counting the colonies using a total plate count. Determination of the antibacterial activity of L. bulgaricus using paper disc diffusion method. SIO made using cold pressed method. The physicochemical properties of SIO were free fatty acids (4.34%), peroxide value (18.13 meq/kg), refractive index (1.4620), and saponification value (187.70 mg KOH/g). The fatty acid components tested using GCMS with the highest concentrations linoleic acid (C18:2) (92.91%) and palmitic acid (C16:0) (7.09%). The SIO concentrations used in the bacterial growth test were 1%, 5%, 10%, 15%. The number of colonies in the four tests was 5.15 x 108, 3.24 x 108, 2.70 x 108, 2.44 x 108 and positive control 5.43 x 108 , negative control 5.36 x 108. The best concentration is 1% concentration and from statistical processing results show that SIO does not significantly stimulate the growth of probiotics. Testing the antibacterial activity of L. bulgaricus with three concentrations (10%, 50%, 100%) and compared with positive controls, namely ciprofloxacin and synbio and negative controls, namely DMSO 10%. The best combination SIO and metabolites was at 100% metabolite concentration with an inhibition zone of 6.33 ± 1.26 mm. The antibacterial activity L. bulgaricus before and after being added with SIO showed a significant difference where the p value <0.05. The result is that the metabolite of L. bulgaricus without added SIO has better antibacterial properties than metabolite with added SIO. Keywords : Antibacterial, Escherichia coli, Lactobacillus bulgaricus, Sacha inchi oil.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Antibakteri, Escherichia coli, Lactobacillus bulgaricus, Minyak sacha inchi
Subjects:	R Medicine > RS Pharmacy and materia medica > RS400-431 Pharmaceutical chemistry
Divisions:	08-Faculty of Mathematics and Natural Science > 48201-Pharmacy (S1)
Depositing User:	Nina Camelia Sembiring
Date Deposited:	09 Aug 2022 07:56
Last Modified:	09 Aug 2022 07:56
URI:	http://repository.unsri.ac.id/id/eprint/76781

Actions (login required)

View Item