Bukti korespondensi: Phylogenetic of Featherback Chitala sp from South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene

Anjarsari, Y and Syaifudin, Mochamad and Jubaedah, Dade and Taqwa, Ferdinand Hukama and Yonarta, Danang (2020) Bukti korespondensi: Phylogenetic of Featherback Chitala sp from South Sumatra Based on Cytochrome C Oxidase Subunit I (COI) Gene. IOP Publishing Ltd., Palembang.

Text (Bukti korespondensi: Phylogenetic of Featherback Chitala IOP) 00_bukti korespondensi Phylogenetic of Featherback Chitala.pdf Download (542kB)

Abstract

The featherback Chitala sp in Indonesia inhabit riverine of Sumatra, Java and Kalimantan. Featherbacks have been protected as threatened species. This research aims to identify the sequence of Cytochrome C Oxidase Subunit I (COI) gene of mtDNA, and construct the phylogenetic trees between species of featherbacks in South Sumatra. This research was conducted in March–August 2020. The methods used in barcoding species and determining phylogenetic i.e., DNA isolation, DNA amplification using PCR and sequencing of COI gene regions of mtDNA. The COI gene was sequenced by PCR (Polymerase Chain Reaction) with optimum annealing temperature 500C for 30s with 35 cycles. After editting, sequence length of the COI gene of featherbacks was 621 base pairs (bp) nucleotide. Based on BLASTanalysis, specimen CLP2 from PT PLN Indralaya had the highest similarity (99.28%) to C. lopis (Malaysia), then 98.88% to the same species from Kampar River, Riau. Speciemens from Musi River (CLS1, CLS 3) and PT PLN (CLP3) indicated the highest similarity of 95.19% with C. chitala from India. The phylogenetic trees showed that Chitala formed four subclusters and it was clearly distant between species C. lopis and C. chitala (bootstrap value =73).

Item Type:	Other
Subjects:	#3 Repository of Lecturer Academic Credit Systems (TPAK) > Corresponding Author
Divisions:	05-Faculty of Agriculture > 54243-Aquaculture (S1)
Depositing User:	Mr. Ferdinand Hukama Taqwa
Date Deposited:	13 May 2023 11:06
Last Modified:	13 May 2023 11:06
URI:	http://repository.unsri.ac.id/id/eprint/102952

Actions (login required)

View Item