PENGARUH PEMBERIAN PLATELET RICH FIBRIN UNTUK MENCEGAH PEMBENTUKAN STRIKTUR ESOFAGUS AKIBAT CEDERA KAUSTIK BASA PADA HEWAN COBA TIKUS: TINJAUAN TERHADAP EKSPRESI Mmp1, Mmp8, Timp1

ZULEIKA, PUSPA and Saleh, Irsan and Murti, Krisna and Liberty, Iche Andriyani (2024) PENGARUH PEMBERIAN PLATELET RICH FIBRIN UNTUK MENCEGAH PEMBENTUKAN STRIKTUR ESOFAGUS AKIBAT CEDERA KAUSTIK BASA PADA HEWAN COBA TIKUS: TINJAUAN TERHADAP EKSPRESI Mmp1, Mmp8, Timp1. Doctoral thesis, Sriwijaya University.

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Abstract

Caustic esophageal injury may result in esophageal stricture. In other studies, Mmp1, Mmp8, and Timp1 are related to the pathophysiogical changes observed in esophageal stricture. Several prospective treatments to prevent esophageal stricture have been studied with varying costs and results. Platelet-rich fibrin has been proven to be safe and efficacious to improve tissue regeneration. This study aims to prove PRF’s role in esophageal stricture prevention in the rat model. This study was divided into two phases: validation of a non-invasive rat model exposed to 0.1 mL of 10% NaOH via feeding tube and elucidation of the effect of varying volumes (0.1-0.3 mL) of administered PRF to prevent esophageal stricture on the previously-validated rat model. Platelet-rich fibrin originated from allograft rat donors. Differential expressions of Mmp1, Mmp8, and Timp1 were measured between 1-14 days through standardized immunohistochemical protocols while tissue damage and Stenosis Index were evaluated histologically. This study was able to validate the rat model. Further, this study discovered differential Mmp1 and Timp1 expression after administration while simultaneously discovering a non-significant impact of PRF administration on Mmp8 expression. This study also discovered reduction of histological tissue damage and Stenosis Index after PRF administration. This study discovered the differential role of time, PRF volume administered, Mmp1 and Timp1 expression, and tissue damage in stricture formation. This study discovered that stricture prevention is most significant when PRF was administered at maximum volume (0.3 mL). Keywords: platelet-rich fibrin, esophageal stricture, Mmp1, Mmp8, Timp1, rat model

Item Type:	Thesis (Doctoral)
Uncontrolled Keywords:	Penyembuhan Luka, Esofagitis Korosif, Asam Kuat, Basa Kuat
Subjects:	R Medicine > R Medicine (General) > R690-697 Medicine as a profession. Physicians
Divisions:	04-Faculty of Medicine > 11901-Doctor Profession (Profesi)
Depositing User:	Puspa Zuleika
Date Deposited:	13 Jan 2025 04:43
Last Modified:	13 Jan 2025 04:43
URI:	http://repository.unsri.ac.id/id/eprint/163267

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