Hermansyah, Hermansyah and Susilawati, Susilawati (2021) GENE EXPRESSION CHANGES AND ANTI-PROLIFERATIVE EFFECT OF NONI (Morinda citrifolia)FRUIT EXTRACT ANALYSED BY REAL TIME-PCR (Similarity). Turnitin Universitas sriwijaya. (Submitted)
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Abstract
To elucidate the anti-proliferative effect of noni (Morinda citrifolia) fruit extract for a Saccharomyces cerevisiaemodel organism, analysis of gene expression changes related to cell cycle associated with inhibition effect of noni fruit extract was carried out. Anti-proliferative of noni fruit extract was analyzed using gene expression changes of Saccharomyces cerevisiae(strains FY833 and BY4741). Transcriptional analysis of genes that play a role in cell cycle was conducted by growing cells on YPDAde broth medium containing 1% (w/v) noni fruit extract, and then subjected using quantitative real-time polymerase chainreaction (RT-PCR). Transcriptional level of genes CDC6 (Cell Division Cycle-6), CDC20 (Cell Division Cycle-20), FAR1 (Factor ARrest-1), FUS3 (FUSsion-3), SIC1 (Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase-1), WHI5 (WHIskey-5), YOX1 (Yeast homeobOX-1)and YHP1(Yeast Homeo-Protein-1) increased, oppositelygenes expression ofDBF4 (DumbBell Forming), MCM1 (Mini Chromosome Maintenance-1)andTAH11 (Topo-A Hypersensitive-11) decreased, while the expression level of genes CDC7 (Cell Division Cycle-7), MBP1 (MIul-box Binding Protein-1)and SWI6 (SWItching deficient-6)relatively unchanged. These results indicated that gene expression changes might associate with anti-proliferative effect from noni fruit extract. These gene expressions changes lead to the growth inhibition of S.cerevisiaecell because of cell cycle defect.
| Item Type: | Other |
|---|---|
| Subjects: | Q Science > QD Chemistry > QD415-436 Biochemistry #3 Repository of Lecturer Academic Credit Systems (TPAK) > Results of Ithenticate Plagiarism and Similarity Checker |
| Divisions: | 08-Faculty of Mathematics and Natural Science > 47201-Chemistry (S1) |
| Depositing User: | Hermansyah Hermansyah |
| Date Deposited: | 27 Sep 2021 02:14 |
| Last Modified: | 27 Sep 2021 02:14 |
| URI: | http://repository.unsri.ac.id/id/eprint/54759 |
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