KINETIKA HIDROLISIS ENZIMATIK DARI SELULOSA SEKAM PADI YANG DIBERI PRAPERLAKUAN HIDROGEN PEROKSIDA-AQUEOUS AMMONIA

EFRINALIA, WINTA and Novia, Novia and Melwita, Elda (2022) KINETIKA HIDROLISIS ENZIMATIK DARI SELULOSA SEKAM PADI YANG DIBERI PRAPERLAKUAN HIDROGEN PEROKSIDA-AQUEOUS AMMONIA. Undergraduate thesis, Sriwijaya University.

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Abstract

Rice husks contain cellulose as a raw material for manufacturing second-generation bioethanol. Cellulose from pre-treated rice husks was converted into reducing sugars through enzymatic hydrolysis using enzymes derived from Aspergillus niger. This study aims to determine the effect of variations in concentration, hydrolysis time and kinetics of enzymatic hydrolysis at enzyme concentrations of 10, 15, and 20% (v/w) and hydrolysis times of 5, 10, 15, 20, and 25 h. The results showed that cellulose was hydrolyzed to form reducing sugars. The CMCase activity and FPase activity reached 548.940 and 314.892 U mL-1, respectively, much higher than most previous reports on this genus. From the calculation of the reaction rate using the Michaelis–Menten kinetic model, the value of the Michaelis constant ranges from 0.001 to 0.0007, and the maximum rate is 1.3 × 10-7 to 2.7 × 10-7 Mol L-1 s-1. The highest reducing sugar concentration was obtained (1.80 g L-1) at an enzyme concentration of 20% (v/w) and a hydrolysis time of 25 h with an optimal condition pH of 5.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	hidrolisis enzimatik; model kinetik; Michaelis–Menten; sekam padi yang sudah diolah sebelumnya; gula pereduksi
Subjects:	T Technology > TP Chemical technology > TP500-660 Fermentation industries. Beverages. Alcohol
Divisions:	03-Faculty of Engineering > 24101-Chemical Engineering (S2)
Depositing User:	Winta Efrinalia
Date Deposited:	01 Dec 2022 05:57
Last Modified:	01 Dec 2022 05:57
URI:	http://repository.unsri.ac.id/id/eprint/83197

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