EKSTRAKSI, PURIFIKASI PARSIAL DAN AKTIVITAS ANTIOKSIDAN SENYAWA TANIN TUMBUHAN APU-APU (PISTIA STRATIOTES)

SITUNGKIR, LOUSIANA JUNI ARTHA and Sudirman, Sabri (2023) EKSTRAKSI, PURIFIKASI PARSIAL DAN AKTIVITAS ANTIOKSIDAN SENYAWA TANIN TUMBUHAN APU-APU (PISTIA STRATIOTES). Undergraduate thesis, Sriwijaya University.

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Abstract

This study aimed to measure the content of tannin compounds and antioxidant activity between crude extract and purified extracts on water lettuce (Pistia stratiotes). In this study, identification of functional groups was also carried out using FT-IR (Fourier Transform Infra Red). This research method was carried out experimentally in a laboratory using one treatment consisting of 2 levels, namely crude extract and purified extract and was repeated 3 times. The data obtained were analyzed quantitatively, then continued by using different test parametric statistics (Independent sample t-test). The test parameters included extract yields, total tannin, analysis of antioxidant activity, and identification of FT-IR. The test result showed that the crude extract yield was 14,46±1,07% and the purified extract was 5,91±0,57%. The total tannins in each extract were 39,86±1,12 mg TAE/g dry sample and 55,34±1,12 mg TAE/g dry sample. The IC50 values of the two extract were 237±9,39 ppm and 82±3,95 ppm respectively. Identification of the functional groups from the crude extract of the water lettuce contained the functional groups O-H, X=C-Y, N-H, C-N, C-O, and C-X. While the identification of functional groups from the purified extract of the water lettuce contained the functional groups O-H, N-H, and C-O. The O-H functional group is a functional group which indicates that there are tannins (polyphenol) present in the water lettuce extract. The tannins of the water lettuce extract can function as natural antioxidants so the more tannins, the antioxidant activity will increase.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Antioksidan, Ekstrak Kasar, Ekstrak Purifikasi, FT-IR, Tanin
Subjects:	S Agriculture > SH Aquaculture. Fisheries. Angling > SH201-399 Fisheries S Agriculture > SH Aquaculture. Fisheries. Angling > SH334.5-334.7 Fishery technology
Divisions:	05-Faculty of Agriculture > 54244-Fisheries Product Technology (S1)
Depositing User:	Lousiana Juni Artha Situngkir
Date Deposited:	28 Jul 2023 03:16
Last Modified:	28 Jul 2023 03:16
URI:	http://repository.unsri.ac.id/id/eprint/123211

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