UJI EFEKTIFITAS NATRIUM HIPOKLORIT (NaOCI) DALAM MENGURANGI TINGKAT KONTAMINASI EKSPLAN TULANG DAUN DURIAN (Durio zibethinus) SECARA IN-VITRO

TRIMALASARI, DESI and Irmawati, Irmawati (2025) UJI EFEKTIFITAS NATRIUM HIPOKLORIT (NaOCI) DALAM MENGURANGI TINGKAT KONTAMINASI EKSPLAN TULANG DAUN DURIAN (Durio zibethinus) SECARA IN-VITRO. Undergraduate thesis, Sriwijaya University.

	Text RAMA_54204_05091182126007.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (3MB) | Request a copy
	Text RAMA_54204_05091182126007_TURNITIN.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (14MB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_01_front_ref.pdf - Accepted Version Available under License Creative Commons Public Domain Dedication. Download (1MB)
	Text RAMA_54204_05091182126007_0020098307_02.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (498kB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_03.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (416kB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_04.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (460kB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_05.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (282kB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_06_ref.pdf - Bibliography Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (245kB) | Request a copy
	Text RAMA_54204_05091182126007_0020098307_07_lamp.pdf - Accepted Version Restricted to Repository staff only Available under License Creative Commons Public Domain Dedication. Download (1MB) | Request a copy

Abstract

Durian (Durio zibethinus Murr.) is a tropical fruit plant native to mainland Southeast Asia. Plant tissue culture is a plant propagation technology from cells, tissues and plant organs in solid or liquid media under aseptic conditions. This study was aimed to evaluate the effectiveness of several sterilization methods, as well as to obtain the best sterilization procedure for durian leaf midrib explants in tissue culture activities with the addition of sodium hypochlorite compounds. This research was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Sriwijaya University, Bukit Lama, Ilir Barat I District, Palembang City, South Sumatra with location coordinates 2o59'23.4''S 104o43'53.4''E. The research period started from June to September 2024. This sterilization treatment used a combination of sterilant ingredients, namely 70% alcohol, bactericide, fungicide, liquid detergent, and NaOCI. Data presentation used parametric descriptive analysis by calculating the percentage of live explants, contaminated explants and contaminated media. The results of this research were the sterilant combination treatment 5(P5), namely liquid detergent for 3 minutes, 0.2% bactericide for 10 minutes, 0.2% fungicide for 10 minutes, 1.5% NaOCI for 5 minutes and 70% alcohol for 5 minutes (54%) showed the best result and corsidered more effective (54%) with the highest percentage of live explants and a fairly low percentage of explant contamination compared to other treatments.

Item Type:	Thesis (Undergraduate)
Uncontrolled Keywords:	Eksplan Tulang Daun Durian, Kultur Jaringan, dan Sterilisasi
Subjects:	S Agriculture > S Agriculture (General) > S1-(972) Agriculture (General) S Agriculture > S Agriculture (General) > S539.5-542.3 Research. Experimentation S Agriculture > S Agriculture (General) > S583-587.73 Agricultural chemistry. Agricultural chemicals S Agriculture > S Agriculture (General) > S602.5-604.37 Methods and systems of culture. Cropping systems. Including fallowing, rotation of crops, plowing S Agriculture > SB Plant culture > SB354-402 Fruit and fruit culture
Divisions:	05-Faculty of Agriculture > 54204-Agronomy (Agricultural Sciences) (S1)
Depositing User:	Desi Trimalasari
Date Deposited:	17 Jan 2025 02:51
Last Modified:	17 Jan 2025 02:51
URI:	http://repository.unsri.ac.id/id/eprint/164928

Actions (login required)

View Item